Plastidial expression of 3?-hydroxysteroid dehydrogenase and progesterone 5?-reductase genes confer enhanced salt tolerance in tobacco

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dc.authorid0000-0001-6767-2539en_US
dc.authorid0000-0002-6591-6007en_US
dc.authorid0000-0003-2933-5296en_US
dc.authorid0000-0003-2976-7224en_US
dc.authorid0000-0002-6786-3973en_US
dc.authorid0000-0001-6262-2866en_US
dc.contributor.authorSameeullah, Muhammad
dc.contributor.authorYıldırım, Muhammet
dc.contributor.authorAslam, Noreen
dc.contributor.authorBaloğlu, Mehmet Cengiz
dc.contributor.authorYücesan, Buhara
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2023-06-26T07:45:35Z
dc.date.available2023-06-26T07:45:35Z
dc.date.issued2021en_US
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.descriptionThis research was funded by the Scientific and Technological Research Council of Turkey (TUBITAK), grant number KBAG-115Z470 to E.G.en_US
dc.description.abstractThe short-chain dehydrogenase/reductase (SDR) gene family is widely distributed in all kingdoms of life. The SDR genes, 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and progesterone 5-beta-reductases (P5 beta R1, P5 beta R2) play a crucial role in cardenolide biosynthesis pathway in the Digitalis species. However, their role in plant stress, especially in salinity stress management, remains unexplored. In the present study, transplastomic tobacco plants were developed by inserting the 3 beta-HSD, P5 beta R1 and P5 beta R2 genes. The integration of transgenes in plastomes, copy number and transgene expression at transcript and protein level in transplastomic plants were confirmed by PCR, end-to-end PCR, qRT-PCR and Western blot analysis, respectively. Subcellular localization analysis showed that 3 beta-HSD and P5 beta R1 are cytoplasmic, and P5 beta R2 is tonoplast-localized. Transplastomic lines showed enhanced growth in terms of biomass and chlorophyll content compared to wild type (WT) under 300 mM salt stress. Under salt stress, transplastomic lines remained greener without negative impact on shoot or root growth compared to the WT. The salt-tolerant transplastomic lines exhibited enhanced levels of a series of metabolites (sucrose, glutamate, glutamine and proline) under control and NaCl stress. Furthermore, a lower Na+/K+ ratio in transplastomic lines was also observed. The salt tolerance, mediated by plastidial expression of the 3 beta-HSD, P5 beta R1 and P5 beta R2 genes, could be due to the involvement in the upregulation of nitrogen assimilation, osmolytes as well as lower Na+/K+ ratio. Taken together, the plastid-based expression of the SDR genes leading to enhanced salt tolerance, which opens a window for developing saline-tolerant plants via plastid genetic engineering.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)en_US
dc.identifier.citationSameeullah, M., Yildirim, M., Aslam, N., Baloğlu, M. C., Yucesan, B., Lössl, A. G., ... & Gurel, E. (2021). Plastidial Expression of 3β-Hydroxysteroid Dehydrogenase and Progesterone 5β-Reductase Genes Confer Enhanced Salt Tolerance in Tobacco. International Journal of Molecular Sciences, 22(21), 11736.en_US
dc.identifier.doi10.3390/ijms222111736
dc.identifier.endpage23en_US
dc.identifier.issn1422-0067
dc.identifier.issue21en_US
dc.identifier.pmid34769166en_US
dc.identifier.scopus2-s2.0-85118120990en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttp://dx.doi.org/10.3390/ijms222111736
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11209
dc.identifier.volume22en_US
dc.identifier.wosWOS:000720036600001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSameeullah, Muhammad
dc.institutionauthorYıldırım, Muhammet
dc.institutionauthorAslam, Noreen
dc.institutionauthorYücesan, Buhara
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofInternational Journal of Molecular Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectShort Chain Dehydrogenaseen_US
dc.subjectReductase (SDR) Genesen_US
dc.subjectTransplastomic Plantsen_US
dc.subjectSalt Toleranceen_US
dc.subject3β -HSDen_US
dc.subjectNMRen_US
dc.subjectSucroseen_US
dc.titlePlastidial expression of 3?-hydroxysteroid dehydrogenase and progesterone 5?-reductase genes confer enhanced salt tolerance in tobaccoen_US
dc.typeArticleen_US

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