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Öğe Molecular phylogeny of plant pathogenic fungi based on start codon targeted (SCoT) polymorphism(Springer, 2023) Palacıoğlu, Gülsüm; Alkan, Mehtap; Derviş, Sibel; Bayraktar, Harun; Özer, GökselBackgroundA number of molecular marker systems have been developed to assess genetic diversity, carry out phylogenetic analysis, and diagnose and discriminate plant pathogenic fungi. The start codon targeted (SCoT) markers system is a novel approach used here to investigate intra and interspecific polymorphisms of phytopathogenic fungi.Materials and methodsThis study assessed genetic variability between and within 96 isolates of ten fungal species associated with a variety of plant species using 36 SCoT primers.ResultsThe six primers generated 331 distinct and reproducible banding patterns, of which 322 were polymorphic (97.28%), resulting in 53.67 polymorphic bands per primer. All primers produced informative amplification profiles that distinguished all fungal species. With a resolving power of 10.65, SCoT primer 12 showed the highest polymorphism among species, followed by primer 33 and primer 29. Polymorphic loci (PPL), Nei's diversity index (h), and Shannon index (I) percentages were 6.25, 0.018, and 0.028, respectively. UPGMA analysis separated all isolates based on morphological classification and revealed significant genetic variation among fungal isolates at the intraspecific level. PCoA analysis strongly supported fungal species discrimination and genetic variation. The other parameters of evaluation proved that SCoT markers are at least as effective as other DNA markers.ConclusionsSCoT markers were effective in identifying plant pathogenic fungi and were a powerful tool for estimating genetic variation and population structure of different fungi species.Öğe Graph-based algorithm for the understanding of failures in the ATLAS infrastructure(IOP Publishing, 2023) Uribe, Gustavo A.; Tortajada, Ignacio Asensi; Sanchez, Carlos Solans; Rummler, Andre; Oyulmaz, Kaan Yüksel; Denizli, HalukThe ATLAS Technical Coordination Expert System is a knowledge-based application which describes and simulates the ATLAS experiment based on its components and their relationships with differing levels of granularity but with an emphasis on general infrastructure. It facilitates the sharing of knowledge and improves the communication among experts with different backgrounds and domains of expertise. The developed software has become essential for the planning of interventions as it gives easily insight into their consequences. Furthermore, it has also proven to be useful for exploring the most effective ways to improve the ATLAS operation and reliability by identifying points of failure with significant impact. The underlying database describes more than 13,000 elements with 89,000 relationships among them. It combines information from diverse domains such as detector control and safety systems, gas and water supplies, cooling, ventilation, cryogenics, and electricity distribution. As the most recent addition, a tool to identify the most probable cause of a failure state has been developed. This paper discusses the graph-based algorithm currently implemented by that tool and shows its behaviour based on the parameters entered by the user. An example in form of a real failure event is given which demonstrates the potential of the Expert System for understanding major failures faster in urgent situations.Öğe Prevalence of Nosemosis and Varroosis in honey bees in Sinop province(Kastamanu University, 2023) Yaman, Mustafa; Güvendik, Tuğba Sağlam; Ünal, SabriAbstract Aim of study: Sinop province has a potential to progress in beekeeping. In this study, it was aimed to investigate the presence and distribution of nosemosis and varroosis in honey bees in Sinop province. Area of study: 12 villages from 7 districts (Ayancık, Boyabat, Dikmen, Durağan, Erfelek, Gerze and Saraydüzü) in Sinop province were determined as study area. Material and methods: A total of 874 adult bee samples were dissected in Ringer’s solution and examined for nosemosis. A total of 1.640 adult bee samples were examined for varroosis. Main results: Nosemosis was the most common disease. Average of the nosemosis infection was 7.6%. On the other hand, varroosis was observed in the eight of 12 localities examined in Sinop province. Total infestation ranged from 0.6 to 8.2%. Highlights: Nosemosis and varroosis were observed in eight (66.7%) of the 12 examined localities in Sinop province. These data are considered as proof that honey production is negatively affected. This study also confirms that the beekeepers in the Sinop province should be informed in detail about the precautions to be taken in the monitoring and controlling varroosis and nosemosis.Öğe Action myoclonus-renal failure syndrome: A case report with bioinformatic annotations(Springernature, 2023) Ekmekçi, Hakan; Qutob, Omar; Babayev, Huseyn; Şahin, AliAction myoclonus-renal failure (AMRF) syndrome is a rare autosomal recessive disorder characterized by myoclonic epilepsy with occasional renal failure comorbidity. This study examines a consanguineous family with multiple members presenting myoclonic epilepsy. The disease's continued transmission within the family is attributable to a lack of genetic testing and the inability to establish a definitive diagnosis. Our objective is to guide physicians toward accurate diagnoses and reduce the disease's recurrence through appropriate genetic counseling. Various diagnostic approaches can contribute to identifying AMRF. While magnetic resonance imaging (MRI) results and blood panels may not yield definitive diagnoses, electromyography (EMG) studies can serve as a robust diagnostic tool, leading to genetic confirmation. In line with standardized protocols, EMG findings consistent with AMRF present a polyneuropathy characterized by axonal degeneration and demyelinating features. These features manifest as decreased amplitude for axonal degeneration and decreased nerve conduction velocity (NCV) for demyelination. The presence of such EMG findings in a patient exhibiting both renal and central nervous system involvement may reinforce a preliminary diagnosis and warrant further genetic analysis.Öğe Perineal care incontinence training for caregivers: Randomised controlled trial(BMJ Publishing Group, 2023) Uzun, Lütfiye Nur; Tok, Hümeyra HançerObjectives The aim of this study was to implement training to increase the perineal care knowledge of relatives caring for incontinent palliative care patients and to examine the effectiveness of this training.Methods This study was designed as a pretest-post-test randomised controlled trial. The study included 84 relatives of patients with incontinence (experimental group, n=42; control group, n=42). The experimental group was given one-on-one face-to-face training on perineal care for patients with incontinence, while the control group was given training through a brochure. Data were collected using a personal information form and the Perineal Care Knowledge Test. The Wilcoxon signed-rank and Mann-Whitney U tests were used for statistical analysis.Results The difference between the pretest and post-test scores of the experimental group was found to be statistically significant (p<0.05), while the difference between the pretest and post-test scores of the control group was not significant (p>0.05). The post-test scores of the experimental group were higher than those of the control group (p<0.05).Conclusions It was found that one-on-one face-to-face perineal care training was a more effective method for increasing the perineal care knowledge of patients' relatives than brochures.Öğe Properties of the uniaxial and biaxial nematic phases of the dodecyltrimethylammonium bromide/1-dodecanol/water lyotropic mixture including azo dye and drug molecules(Taylor & Francis Ltd, 2024) Akpınar, Erol; Tabak, Merve; Reis, Dennys; Neto, Antonio Martins FigueiredoLyotropic nematic phase properties of novel mixtures of dodecyltrimethylammonium bromide (DTMABr)/1-dodecanol (DDeOH)/water, doping with anionic azo dyes (amaranth and tartrazine) and a drug molecule disodium cromoglycate (cromolyn, DSCG), were investigated. The textures of the uniaxial and biaxial nematic phases were observed by polarizing optical microscopy (POM). Uniaxial-to-biaxial nematic phase transitions were determined from the temperature dependence of the birefringences via laser conoscopy. Partial phase diagrams of the mixtures were constructed as a function of concentrations of dyes and drug molecules by combining the POM and laser conoscopy results. Small-angle X-ray scattering (SAXS) was performed to evaluate micellar structure parameters. The results indicate that those dyes and drug molecules have a greater effect on (a) the nematic-nematic phase transition temperatures, (b) the biaxial-nematic phase-domain range in the partial phase diagrams, and (c) micelle-shape anisotropy, comparing to the addition of other conventional inorganic electrolyte ions. Furthermore, dye and drug molecules may be sequenced in the Hofmeister series of ions, considering the number of ionic groups in their molecular structures and also the chaotropic and/or kosmotropic degrees of the ionic groups. Since these molecules may have a resonance structure (e.g. DSCG), as a result of the existence of the aromatic parts in their structures, this resonance structure should be considered to investigate their effects on the stabilization of the lyotropic nematic phases.Öğe A novel blight and root rot of chickpea: A new host for Neoscytalidium dimidiatum(Elsevier Science Ltd, 2023) Güney, İnci Güler; Bozoğlu, Tuğba; Özer, Göksel; Derviş, SibelIn the southeastern province of Mardin in Turkey, a severe and novel fungal infection affected all chickpea plant parts, resulting in blight symptoms on stem, petiole, branch, and leaf, defoliation, seed and root rot, and death. Neoscytalidium dimidiatum was identified as the agent responsible for this new blight and root rot using sequence analysis of the ITS, tef1, and tub2 loci as well as morphological data. The pathogen was found in all surveyed districts and fields, with varying incidences of blight and root rot, blight being nearly twice (40%) as common as root rot (21%), and root-rotted and blighted plants co-occurring in the majority of instances. All 92 N. dimidiatum isolates from various tissues induced necrotic lesions on the inoculated plants, indicating they were pathogenic for chickpea. Conidia exhibited germination across a temperature range, with increasing temperatures positively influencing germination rates, and mycelial growth was significantly influenced by temperature, with the optimal growth temperature observed to be 35 degrees C. The response of 25 Turkish chickpea cultivars and three other genotypes when subjected to inoculation with Ciar 12 and Ciar 78 isolates, representative of phylogenetic clusters, was evaluated based on the severity of blight and root rot. The majority of cultivars and genotypes displayed high susceptibility and suffered mortality when exposed to either soil or spray inoculation with each isolate. Among the assessed cultivars and genotypes, cagatay had the lowest severity of root rot. This study is the first to report a natural infection of chickpea plants by N. dimidiatum. Under global warming, this may be detrimental to chickpea cultivation and habitat in southeastern Turkey, which is the origin of chickpeas.Öğe Evaluation of the effect of COVID-19 on static balance in healthy young individuals(Bayrakol Medical Publisher, 2023) Şenol, Gamze Taşkın; Kürtül, İbrahim; Ray, Abdullah; Ahmetoğlu, GülçinAim: It is known that COVID-19 infection has various physiological effects. And, it also has negative effects on the balance. This study focused on evaluating the static balance of healthy individuals who either had or did not have a history of COVID-19.Material and Methods: The study included 30 individuals who were previously diagnosed with COVID-19 infection (positive PCR test), who recovered later on, and 30 individuals as a control group. After the dominant foot of both groups was determined, the flamingo balance test was used to evaluate static balance, and Dizziness Handicap Inventory (DHI) was applied to the group that had a COVID-19 infection history.Results: A significant difference was found between dominant foot balance and non-dominant foot balance in individuals who had COVID-19 and in the control group.Discussion: The severity of recent cases of COVID-19 disease that affect the balance system has risen significantly. This study showed that individuals with COVID-19 have problems with static balance compared to those without COVID-19. In our estimation, post-recovery rehabilitation programs for people who have had COVID-19 should include balancing exercises.Öğe The relationship between attitudes toward technology and openness to innovation of academicians teaching music remotely(Hacettepe University, 2023) Çocar, Ebru; Kahraman, Ekin ÇoraklıThe present study aimed to determine whether there is a correlation between the attitudes of music academicians who teach music remotely toward technology and their level of openness to innovation, and to examine if these attitudes and levels vary according to certain variables. The study sample was composed of a total of 74 music academicians working in the faculties of education, conservatories, and faculties of fine arts of universities in various regions in Turkey in the academic year of 2020-2021. Using relational screening model, the present study compiled data through the 'Attitudes toward Technology Scale by Akbaba Altun (2003), and the Characteristics of Innovative Teachers Scale of Kocasarac (2018). Pearson Correlation Analysis was employed to determine the relationship between academicians' attitudes toward technology and their levels of openness to innovation, and these attitudes and levels were analysed according to certain variables through t -test, Mann-Whitney U, Kruskal-Wallis, and ANOVA test methodologies. The findings indicated a high positive correlation between the attitudes toward technology and levels of openness to innovation of academics teaching music remotely. In addition, it was found that academicians' levels of openness to innovation and attitudes toward technology did not significantly differ according to gender, level of education, and their fields of expertise.Öğe Stable measurement system for platinum resistance temperature detector(Maik Nauka/Interperiodica/Springer, 2023) Altınkaya, Serdar; Bayrak, Alper; Daldal, Nihat; Özdil, Osman Eren-Stable and precise temperature measurement is crucial for thermodynamic applications. The stability and precision of the measurement depend on the sensor type and measurement method. In industrial applications, platinum resistance temperature detectors (RTD) are widely preferred. In this study, stable and precise temperature measurement methods by using the Platinum RTD PT1000 sensor are investigated. Measurement methods are considered as measurement circuits and digital filter applications, separately. Experimental studies were evaluated on a household-type oven and comparative results are presented.Öğe Optimization and characterization of biodegradable films from chicken gelatin crosslinked with oxidized phenolic compounds(Elsevier, 2024) Erge, Aydın; Güler, Büşra Zeynep; Eren, ÖmerChicken gelatin derived from poultry by-product was combined with caffeic acid (CA), rutin (RUT) and glycerol (GLY) to obtain biodegradable films. Optimum cross-linking conditions were investigated using Response Surface Methodology (RSM). The results showed that cross-linking led to lower L* value and higher b*, and the higher opacity values in the films. Water solubility (WS) decreased up to 50% after the incorporation of 1.25% CA compared to the commercial gelatin (cattle and pig based) films. Crosslinking improved the thermal stability and the tensile strength (TS) of films. Optimized cross-linking combination was determined as 0.96-1.56% CA, 0-1.25% RUT, and 29.5-30.5% GLY. Overall, this study demonstrated that crosslinking by CA and RUT can be used to improve the physical and barrier properties of gelatin films having excellent potential for the development of biodegradable films for packaging uses. These films may also result in an improvement and added value in poultry by-products.Öğe Sex determination by the machine learning algorithms through using morphometric measurements of the carpal, metacarpal, and phalangeal bones(Sociedad Chilena de Anatomía, 2023) Şenol, Gamze Taşkın; Kürtül, İbrahim; Ray, Abdullah; Ahmetoğlu, GülçinIn the study, it was aimed to predict sex from hand measurements using machine learning algorithms (MLA). Measurements were made on MR images of 60 men and 60 women. Determined parameters; hand length (HL), palm length (PL), hand width (HW), wrist width (EBG), metacarpal I length (MIL), metacarpal I width (MIW), metacarpal II length (MIIL), metacarpal II width (MIIW), metacarpal III length (MIIL), metacarpal III width (MIIIW), metacarpal IV length (MIVL), metacarpal IV width (MIVW), metacarpal V length (MVL), metacarpal V width (MVW), phalanx I length (PILL), measured as phalanx II length (PIIL), phalanx III length (PIIL), phalanx IV length (PIVL), phalanx V length (PVL). In addition, the hand index (HI) was calculated. Logistic Regression (LR), Random Forest (RF), Linear Discriminant Analysis (LDA), K-nearest neighbour (KNN) and Naive Bayes (NB) were used as MLAs. In the study, the KNN algorithm's Accuracy, SEN, F1 and Specificity ratios were determined as 88 %. In this study using MLA, it is understood that the highest accuracy belongs to the KNN algorithm. Except for the hand's MIIW, MIIIW, MIVW, MVW, HI variables, other variables were statistically significant in terms of sex difference.Öğe Quantum coherence and non-Markovianity in a noisy quantum tunneling problem(Springer, 2023) Mahdi, Nisreen Mohammed; Kurt, Arzu; Altıntaş, FerdiWe investigate the coherence and non-Markovianity of a quantum tunneling system whose barrier is fluctuated by a telegraph noise, and its energy gap is modulated by Gaussian noise. With the help of averaging method, the system dynamics are analytically derived, and the analytical expression for coherence measure and non-Markovianity, focusing on a narrow range of parameter regimes for both initially coherent and non-coherent states are obtained. We observe non-Markovian dynamics in a situation where the Kubo number is high. It is also found that there is no strong relation between the coherence of the system and non-Markovian dynamics except a region in which these two tend to change their behavior at the intermediate noise color for two initial states.Öğe Evolution of basic features of Bi1.8Pb0.4Sr2Ca2.2Cu3Oy ceramic systems with NiO impurity(Springer, 2023) Kara, Emre; Doğan, Muhsin Uğur; Kaya, Şenol; Terzioğlu, Rıfkı; Yıldırım, Gürcan; Terzioğlu, CabirIn the current work, we have investigated the role of different nickel oxide (NiO)(x) impurity addition levels (0 & LE; x & LE; 1.2) on the fundamental crystallographic, morphological, electrical, magnetic, critical current density, and superconducting features of Bi1.8Pb0.4Sr2Ca2.2Cu3Oy (Bi-2223) by the temperature-dependent electrical resistivities (& rho;-T), X-ray diffraction data (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and magnetic hysteresis (M-H) measurements for the first time. The NiO-added Bi-2223 ceramics have been produced by the standard solid-state reaction method at 840 & DEG;C for 36 h in the air atmospheric conditions. EDX investigations have demonstrated that there appear no extra contamination and additional phases for any anionic or cation-including phases. Besides, the Ni impurity concentration has been observed to increase systematically depending on the impurity amount. Thus, the NiO impurities have been added to the ceramic matrix as requested. Similar findings have been confirmed by the XRD examinations. The experimental results have pointed out the multiple substitution reaction of Ni-sites with the Bi-sites, Ca-sites, and Cu-sites in the Bi-2223 system. Moreover, the experimental results have shown that all the fundamental features are strongly dependent upon the NiO addition level due to the variation of Cu-O-2 interlayer bonding forces, grain alignment distributions, mobile carrier concentrations in the in-plane Cu-O-2 layer x(2)-y(2) bands, and coupling probabilities between the adjacent layers in the crystal structure. Similarly, the induction of new impurity phase formations, grain boundaries, modulation of Bi-O double layers, spin fluctuations, inhomogeneities, structural defects, and non-recoverable structural problems have also triggered the decrease in the main characteristic quantities. On this basis, the microstructural morphology and related crystallographic features have been noted to degrade with the impurity. Furthermore, the presence of NiO impurity has resulted in a decrement in the flux-pinning centers due to the increase in the lower resistance regions against the flux drifts in the bulk crystal system. All in all, the addition of NiO impurity in the Bi-2223 ceramic matrix is not a good idea for the improvement in the fundamental features of the Bi-2223 superconducting structure.Öğe MALTA monolithic pixel sensors in TowerJazz 180 nm technology(Elsevier, 2023) Sanchez, C. Solans; Allport, P.; Denizli, Haluk; Berlea, D. V.; Oyulmaz, Kaan Yüksel; Bortoletto, D.Depleted Monolithic Active Pixel Sensors are of highest interest at the HL-LHC and beyond for the replacement of the Pixel trackers in the outermost layers of experiments where the requirement on total area and cost effectiveness is much bigger. They aim to provide high granularity and low material budget over large surfaces with ease of integration. Our research focuses on MALTA, a radiation hard DMAPS with small collection electrode designed in TowerJazz 180 nm CMOS imaging technology and asynchronous read-out. Latest prototypes are radiation hard up to 2 x 1015 1 MeV neq/cm2 with a time resolution better than 2 ns.Öğe Meta-analysis of sugar beet (Beta vulgaris L.) transcriptome profiles under different biotic and abiotic stress conditions(Springer, 2023) Bulut, Burak; Gürel, Songül; Ünüvar, Ömer Can; Gürel, Ekrem; Ünlü, Ercan SelçukSugar beet (Beta vulgaris L.) meets the 21% of world sugar production. Soil pollution, biotic and abiotic factors in production areas greatly reduce product quantity and quality. Sugar beet responds to biotic and abiotic stresses such as drought, salt, heat, light, and infections of nematode, bacteria and fungi at the molecular level. Understanding molecular mechanisms require comprehensive genomics studies in order to control these mechanisms to increase the yield and quality. Transcriptome studies performed under stress conditions can shed light on the responses of plants at the molecular level. In addition, meta-analysis can help to find common responses under different stress conditions. In this study four different stress-related transcriptome data were used: two of them are related with biotic stress (nematode and fungi infection) and two of them are related with abiotic stress (ABA treatment and salt stress). In this study, we performed meta-analysis of studies conducted under biotic and abiotic stress conditions. Our results revealed 460 commonly regulated genes from biotic stress related data and 1031 commonly regulated genes from abiotic stress related data. Our data also showed that expression of ten genes is controlled regardless of the type of stress condition. The data can be useful for understanding the molecular aspect of adaptive stress response in sugar beet.Öğe Synthesis, crystal structure, DFT, Hirshfeld surface analysis, energy frameworks and in-silico drug-targeting PFKFB3 kinase of novel triazolequinoxalin derivative (TZQ) as a therapeutic strategy against cancer(Elsevier Sci LTD, 2023) Abad, Nadeem; Al-Ostoot, Fares Hezam; Ashraf, Sajda; Chkirate, Karim; Aljohani, Majed S.; Buhlak, ShafeekOverall, drug design is a dynamic and evolving field, with researchers constantly working to improve their understanding of molecular interactions, develop new computational methods, and explore innovative techniques for creating effective and safe medications. The process can involve steps such as the identification of targets, the discovery of lead compounds, lead optimization, preliminary testing, human trials, regulatory approval and finally post-marketing surveillance, all aimed at bringing a new drug from concept to market. In this article, the synthesis of the novel triazolequinoxalin (TZQ) 1-((1-hexyl-1H-1,2,3-triazol-5-yl)methyl)-3-phenylquinoxalin-2(1H)one (4) is reported. The structure has been identified with a variety of spectroscopic methods (1H, 13C NMR, and LC-MS) and finally, the structure has been determined by X-ray diffraction (XRD) studies. The TZQ molecule has crystallized in the monoclinic space C2/c group with unit cell dimensions a = 41.201(2) angstrom, b = 10.6339(6) angstrom, c = 9.4997(4) angstrom, beta = 93.904(4). The crystal structure is stabilized by intermolecular interactions (N-H MIDLINE HORIZONTAL ELLIPSIS O and N-H ... Cg) occurring within the molecule. The presence of these intermolecular interactions is evaluated through analysis of Hirshfeld surfaces (HS) and two-dimensional (2D) chemical fingerprints map. Additionally, energy frameworks were employed to identify the prevailing interaction energy influencing the molecular arrangement. Density Functional Theory (DFT) calculations were computed toÖğe Sensitivity of anomalous quartic gauge couplings via tri-photon production at FCC-hh(Elseiver, 2024) Şenol, Abdulkadir; Denizli, Haluk; Helveci, CerenA direct investigation of the self-couplings of gauge bosons, completely described by the non-Abelian gauge symmetry of the Standard Model, is extremely valuable in understanding the gauge structure of the SM. Any deviation from the SM predictions on gauge boson self-coupling is to give a hint at the existence of a new physics beyond the SM, which is defined with a modification of the self-interactions using an effective field theory approach. In this paper, we present a detailed Monte Carlo study searching for anomalous quartic gauge dimension-8 couplings related to yyyy and yyyZ vertices at the future hadron-hadron collider (FCC-hh) via tri-photon production at a 100 TeV center of mass energy with an integrated luminosity L1,,, =30 ab-1. Events that have been parton showered and include detector effects are analyzed with a Toolkit for Multivariate Data Analysis (TMVA) using a boosted decision tree to help distinguish between signal and background events to achieve the best sensitivities on anomalous quartic gauge couplings. Our obtained results reveal that the limits on anomalous quartic gauge couplings fT 8/Lambda 4 and fT 9/Lambda 4 at 95% C.L. without systematic errors are about three orders of magnitude stronger compared to the best current experimental limits reported by the ATLAS collaboration at the LHC. Considering a realistic systematic uncertainty such as 10% from possible experimental sources, our obtained limits of anomalous quartic couplings get worse by about one order of magnitude compared to those without systematic uncertainty but are still two orders of magnitude better than those recently reported by ATLAS.Öğe Do vermicompost applications ımprove growth performance, pharmaceutically ımportant alkaloids, phenolic content, free radical scavenging potency and defense enzyme activities in summer snowflake (Leucojum aestivum L.)?(Wiley-VCH Verlag GMBH, 2023) Çimen, Ayça; Baba, Yavuz; Yıldırım, Arzu Birinci; Türker, Arzu UçarLeucojum aestivum L. contains galanthamine and lycorine, which are two pharmaceutically valuable alkaloids. Vermicompost (VC), an organic waste product created by earthworms enhances soil quality and can improve the medicinal quality of the plant that is crucial to the pharmaceutical industry. The aim of this study was to determine the effects of four different VC concentrations (5 %, 10 %, 25 %, and 50 %) on L. aestivum growth parameters, alkaloid levels (galanthamine and lycorine), total phenol-flavonoid content, free radical scavenging potential, and defense enzyme activities (SOD and CAT) compared to control (no VC). The width, length, and fresh weight of the leaves were improved by 10 % VC treatment. The highest total phenolic content was found in the bulbs and leaves treated with 50 % VC. HPLC-DAD analysis of alkaloids showed that 10 % and 50 % VC treatments contained the most galanthamine in the bulb and leaf extracts, respectively. The application of 25 % VC was the most efficient in terms of lycorine content in both extracts. CAT activity was elevated at 10 %, 25 %, and 50 % VC. Based on the growth performance and galanthamine content of the bulbs and leaves, it can be concluded that a 10 % VC application was the most effective in the cultivation of L. aestivum.Öğe Antiepileptic effects of exenatide in penicillin induced acute epilepsy model in rats(The National Institute of Science Communication and Information Resources (NISCAIR), 2023) Yıldız, Ayşegül; Kocacan, Süleyman Emre; Him, AydınGlucagon-like peptide-1 receptors are widely expressed in the brain and its association with nitric oxide is suggestive of its role in epilepsy. In this study, we investigated the effects of exenatide, a glucagon-like peptide-1 receptor agonist, on the epileptiform activity induced by penicillin injection. The study used 72 male Wistar albino rats in 9 groups. All groups except the last group which received only exenatide, received intracortical penicillin injection to induce epileptiform activity. Exenatide was intraperitoneally injected in II-IV groups, at doses of 50, 100, 200 & mu;g/kg, respectively. Sodium nitroprusside (SNP) and Nco-nitro-L-arginine methyl ester (L-NAME) were injected to the V-VIII groups either alone or with exenatide. Electrocorticography was recorded for 3 h. While administration of 200 & mu;g/kg exenatide reduced the frequency of epileptiform activity, 50 and 100 & mu;g/kg doses of exenatide were not effective. When the effective dose of exenatide and the SNP were injected together the spike frequency decreased significantly. When the effective dose of exenatide was given with L-NAME spike frequency significantly decreased only between 90 and 110 min. There was no statistically significant difference in terms of latency and amplitude between the experimental groups. Exenatide had an anticonvulsant effect in penicillin-induced acute epilepsy model which is possibly via nitric oxide and include another pathway since its effect was partially blocked by L-NAME and potentiated by SNP.
