Plastidial expression of 3?-hydroxysteroid dehydrogenase and progesterone 5?-reductase genes confer enhanced salt tolerance in tobacco
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The short-chain dehydrogenase/reductase (SDR) gene family is widely distributed in all kingdoms of life. The SDR genes, 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and progesterone 5-beta-reductases (P5 beta R1, P5 beta R2) play a crucial role in cardenolide biosynthesis pathway in the Digitalis species. However, their role in plant stress, especially in salinity stress management, remains unexplored. In the present study, transplastomic tobacco plants were developed by inserting the 3 beta-HSD, P5 beta R1 and P5 beta R2 genes. The integration of transgenes in plastomes, copy number and transgene expression at transcript and protein level in transplastomic plants were confirmed by PCR, end-to-end PCR, qRT-PCR and Western blot analysis, respectively. Subcellular localization analysis showed that 3 beta-HSD and P5 beta R1 are cytoplasmic, and P5 beta R2 is tonoplast-localized. Transplastomic lines showed enhanced growth in terms of biomass and chlorophyll content compared to wild type (WT) under 300 mM salt stress. Under salt stress, transplastomic lines remained greener without negative impact on shoot or root growth compared to the WT. The salt-tolerant transplastomic lines exhibited enhanced levels of a series of metabolites (sucrose, glutamate, glutamine and proline) under control and NaCl stress. Furthermore, a lower Na+/K+ ratio in transplastomic lines was also observed. The salt tolerance, mediated by plastidial expression of the 3 beta-HSD, P5 beta R1 and P5 beta R2 genes, could be due to the involvement in the upregulation of nitrogen assimilation, osmolytes as well as lower Na+/K+ ratio. Taken together, the plastid-based expression of the SDR genes leading to enhanced salt tolerance, which opens a window for developing saline-tolerant plants via plastid genetic engineering.
Açıklama
This research was funded by the Scientific and Technological Research Council of Turkey (TUBITAK), grant number KBAG-115Z470 to E.G.
Anahtar Kelimeler
Short Chain Dehydrogenase, Reductase (SDR) Genes, Transplastomic Plants, Salt Tolerance, 3β -HSD, NMR, Sucrose
Kaynak
International Journal of Molecular Sciences
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
22
Sayı
21
Künye
Sameeullah, M., Yildirim, M., Aslam, N., Baloğlu, M. C., Yucesan, B., Lössl, A. G., ... & Gurel, E. (2021). Plastidial Expression of 3β-Hydroxysteroid Dehydrogenase and Progesterone 5β-Reductase Genes Confer Enhanced Salt Tolerance in Tobacco. International Journal of Molecular Sciences, 22(21), 11736.
