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Öğe Creation of Turkiye risk map for Cydalima perspectalis (box tree moth) by weighted overlay analysis(Selçuk Ünivesitesi, 2024) Yaman, Şule; Yaman, MustafaBoxwood (Buxus spp.), which is widespread all over the world except Antarctica and widely used in many areas, is threatened by Cydalima perspectalis (box tree moth). Cydalima perspectalis, which has become widespread in Europe, is a new invasive species originating from East Asia and has become widespread in Turkiye in the last ten years. This species was first observed in parks and gardens in Sar & imath;yer, Istanbul, in 2011. Since then, it has continued to spread rapidly in Turkiye. The pest causes intense damage by causing drying on boxwood areas, which causes economic and ecological losses. The rich ecological and topographical conditions of Turkiye are also favorable for the spread of this species. Therefore, it is important to develop pest control methods and take early measures. Within the scope of this study, the aim was to realize early detection of Cydalima perspectalis in Turkiye and make a risk map in this context. For this purpose, a risk map was created by determining the risky areas where Cydalima perspectalis can spread in Turkiye using weighted overlay analysis from geographic information system (GIS) technologies. Existing boxwood locations obtained from field studies were compared with the risk map and analyzed. As a result of the analysis, the presence of Cydalima perspectalis was found in the boxwood in the identified risk areas, and it is expected to be transmitted in boxwood that is in the risk area but not observed to have Cydalima perspectalis. With this research, a risk map for Cydalima perspectalis was made for the first time.Öğe Near-resonant diurnal reactions: A physical model applicable to origin of life processes(Tubitak Scientific & Technological Research Council Turkey, 2012) Budding, Edwin; Akı, Cüneyt; Demircan, Osman; Gündüz, Bülent; Gündüz, Güngör; Özel, Mehmet EminWe adopt that the large residual chemical potential energy (CPE) among reagents of the biosphere constitutes the key physical problem posed by life. We associate the formation of this CPE with the near-resonant behaviour of a two-stage 'A-B' molecular process that behaves as a self-sustaining parametric oscillator. Under suitable conditions, such an oscillator generates CPE when forced by a periodic (daily) insolation. The net growth factor required to explain the current mean excess of biospheric CPE is similar to 5x10(-12) d(-1). This aligns with the mean exponential coefficient of secular oxygen generation in the terrestrial atmosphere. It is also consistent with a feasible scale of oxygen production in certain prebiotic natural photosynthesis scenarios, that can be candidates for the initial A subprocess on the Earth. We schematize initial evolutionary development of the A-B process, including the important role of the intermediate AB compound that provides negative feedback. Supportive C-type molecules also develop as a by-product. The diurnally related distribution of H2O2 on Mars may illustrate a comparable proto-biospheric scheme, and there may be analogous processes on Jupiter. The exponential growth in the lengths of terrestrial nucleotide chain molecules also supports its validity, as does the corresponding growth in measures of cellular complexity. We compare the scenario's implications with biological evidence on the possible co-evolution of blue-light photoreception and circadian timing in Archean photoautotrophs. We consider how a surviving level of cellular organization of circadian rhythmicity, from ancient through to modern times, may be interpreted along these lines, comparing our model with a previously published, comparable, biochemical one.Öğe Description of a new species (Eucypris dorlionensis n. sp.) (Ostracoda, Crustacea) from Türkiye and a cladistic analysis of the genus Eucypris(Tubitak Scientific & Technological Research Council Turkey, 2024) Batmaz, Filiz; Külköylüoğlu, Okan; Yavuzatmaca, MehmetThis is the first clustering analysis of the 53 species of the genus Eucypris where we used 28 plesiomorphic and apomorphic character states in the Winclada/NONA program. Accordingly, Eucypris dorlionensis n. sp. was clustered within Group 2, in which the new species was clearly separated from the other species of the genus based on the shape of the carapace, presence of fine hair -like setae on the carapace surface, the position of the maximum height of the carapace, and length ratio of terminal claws of the second antenna. Eucypris dorlionensis n. sp. was collected from a trough in Eskisehir province in Turkiye where this is the first new species description of the genus that the number of species of the genus has now increased to 10. Besides, the genus is of cosmopolitan distribution known from a variety of aquatic bodies in the world. Including the new species, the number of the species has now raised to 56.Öğe Possible effects of pliocene and pleistocene on the Anatolian populations of andricus tomentosus (Hymenoptera: Cynipidae)(Univ Oradea Publ House, 2023) Mutun, Serap; Danso, Omar A.Andricus tomentosus is an oak gall wasp species widely distributed in the Western Palearctic region and is one of the common parasitic species forming its bell-shaped asexual generation galls on white oak species. Despite its widespread distribution in Turkey, little is known about the species. In this study, we investigated 19 populations of A. tomentosus using partial sequences of the mitochondrial DNA cytochrome b gene and the nuclear ITS2 region to assess the species' genetic diversity and population genetic structure. Two hundred and forty sequences that generated 47 mitochondrial haplotypes and 28 nuclear alleles revealed intermediate to high genetic variation for both studied regions. Some eastern/southeastern Anatolian populations displayed higher diversity than the central/western localities. Demographic analyses and high haplotype/allele versus low nucleotide diversity implied that A. tomentosus populations might have undergone a series of expansion and retraction events in the past, which were correlated with the paleoclimatic and paleoenvironmental changes during Pliocene and Pleistocene. Further, phylogenetic inferences suggested that A. tomentosus populations were divided into two well-diverged clades as eastern and western, which offered that geographic barriers were also the main driver of the lineage formation in A. tomentosus.Öğe Examination of posture and balance in children with primary monosymptomatic nocturnal enuresis(Ios Press, 2023) İnal, Büşra; Özengin, Nuriye; Bakar, Yeşim; Ankaralı, Handan; Öztürk, YusufPURPOSE: This study aimed to examine symptom severity, posture, and balance of children with primary monosymptomatic nocturnal enuresis (PMNE) and compare to a healthy control group. METHODS: Thirty-five children with PMNE and 34 healthy children were included in this study. Physical and sociodemographic characteristics of the children were recorded. Symptom severity was assessed with a Vancouver Non-Neurogenic Lower Urinary Tract Dysfunction/Dysfunctional Elimination Syndrome Questionnaire (NLUTD/DES), a four-day bladder diary and a seven-day bowel diary. Standing postural alignment was assessed with the Spinal Mouse device, and the sensory integration of static balance and dynamic standing balance was assessed with the Biodex Balance System SD. RESULTS: Compared to healthy controls, children with PMNE demonstrated increased symptom severity (p = 0.001), increased upright lumbar lordosis (p = 0.018) and sacral-hip angles (p = 0.029), decreased static balance in the sensory condition of unstable surface with eyes closed (p = 0.001), and decreased mediolateral dynamic balance (p = 0.049). CONCLUSION: Children with PMNE demonstrate altered postural alignment, static and dynamic postural instability, and greater symptom severity on the Vancouver NLUTD/DES than age-matched controls.Öğe Determining diagnostic characteristics of the nonmarine Ostracoda (Crustacea) and their habitat selection with unique functional traits in the Kastamonu province (Türkiye)(Elsevier GmbH, 2024) Külköylüoğlu, Okan; Yavuzatmaca, Mehmet; Tunoğlu, Cemal; Tunçkol, BilgeNonmarine ostracods are widely distributed in a variety of aquatic habitats, from mountain lakes to underground waters, where each species tends to show species-specific habitat preferences with unique functional traits to increase their survival chances. To understand the patterns of such a relationship between habitat preferences and functional traits of the species, 101 different aquatic sites in the Kastamonu province (Türkiye) were randomly sampled during August 2023. A total of 39 ostracods (20 species, 19 unnamed taxa) were encountered from 49 sites. Seventeen species are new reports for the province. Among them, six species are the most common species with cosmopolitan (or cosmoecious) characteristics. The contribution of these species to the diversity of alpha species (p < 0.05) was more than 90 %. The species with relatively high ecological tolerances generally unveiled low-optimum estimates. Canonical correspondence analysis explained 62.5 % of the correlation between environmental variables and species in which redox potential, electrical conductivity, calcium, and pH were found to be the most related variables to the species (p < 0.05). Fundiversity analysis showed that the presence of swimming setae was correlated with high values of functional diversity, functional richness, and functional distribution of species among five elevation ranges. The results of the habitat selection index and the standardized selection index coincided with the functional traits of the most common species. The species characterized by their habitat selection can be considered as diagnostic species of those specific habitats. Among them, Neglecandona neglecta, reported primarily from dams, springs, and troughs, is considered a typical diagnostic species of these habitats, while Cypridopsis vidua found in lakes and dams can be used as an indicator species for these types of lentic habitats. Similarly, three species (Heterocypris incongruens, Ilyocypris bradyi, Psychrodromus olivaceus) can play a critical role as diagnostic species in troughs but reporting them from different habitats such as ditches and rivers / lakes (I. bradyi) and dams (P. olivaceus and H. salina) indicated their wide range of habitat preferences. Hence, the results suggest that the occurrence of diagnostic species seems to correspond to certain habitat types and their functional traits. © 2024 Elsevier GmbHÖğe Updates and improvements of Turkish Plants Data Service (TüBİVES)(2011) Bakış, Yasin; Babaç, Mehmet Tekin; Uslu, EmelReaching biological information of the studied taxa became quite simple by introducing biodiversity information databases through internet. TüBİVES (Turkish Plants Data Service) is the first and the most efficient biodiversity database of the plants in Turkey. In this study, the structural of TüBİVES has been changed according to needs in new data types such as, chromosomal numbers, vernacular names, uses of plants, IUCN categories for endemics, nomenclature and synonym information of plants distributed in Turkey. The structure of database and querying algorithm have been updated both for TüRKNOM (Nomenclatural Database of Turkish Vascular Plants) and TüBİVES. © 2011 IEEE.Öğe Last two hundred individuals: Rediscovery of scilla mesopotamia speta (Hyacinthaceae), a threatened endemic species in Turkey(Polish Botanical Society, 2010) Eker, İsmail; Akan, HasanIn this paper, the rediscovery of endemic and long missing species, Scilla mesopotamica Speta which had been only known from the type gathering, is given. The species has not been collected again after Sintenis in 1888, whose specimens served Speta for description of the new taxon. In 2004, the authors found it in a steppic rocky area, near Halfeti in Şanliurfa province, South East Anatolia, Turkey. After analyzing Sintenis' original collection notes, they concluded that this area is identical with the type locality. 5. mesopotamica has an extremely limited distribution and is represented by less than 200 specimens in the single known population that covers less than 50 m2. Taxonomy of the species, detailed description with illustrations, geographical distribution, habitat, ecology and status of IUCN extinction risk and some comments on conservation of the plant are also presented.Öğe Optimization of morphological data in numerical taxonomy analysis using genetic algorithms feature selection method(Association for Computing Machinery, 2009) Bakış, Yasin; Sezerman, Osman Uğur; Babaç, Mehmet Tekin; Meydan, CemStudies in Numerical Taxonomy are carried out by measuring characters as much as possible. The workload over scientists and labor to perform measurements will increase proportionally with the number of variables (or characters) to be used in the study. However, some part of the data may be irrelevant or sometimes meaningless. Here in this study, we introduce an algorithm to obtain a subset of data with minimum characters that can represent original data. Morphological characters were used in optimization of data by Genetic Algorithms Feature Selection method. The analyses were performed on an 18 character?11 taxa data matrix with standardized continuous characters. The analyses resulted in a minimum set of 2 characters, which means the original tree based on the complete data can also be constructed by those two characters. © 2009 Copyright is held by the author/owner(s).Öğe Functional diversity and functional traits correlate ecology of nonmarine Ostracoda (Crustacea) at different elevational ranges(E Schweizerbartsche Verlagsbuchhandlung, 2024) Külköylüoğlu, Okan; Yavuzatmaca, Mehmet; Özdilek, Ahmet; Tuncer, AlaettinWe tested how functional diversity (FD) along with its three components are correlated to ecological traits of nonmarine ostracods at seven elevational ranges from 800 to 1500 m. 38 ostracod taxa were collected from 55 aquatic sites in Aksaray province (Turkey). 21 species were new reports for the province. Shannon-Wiener diver-sity index indicated four cosmopolitans with more than 90 % of contribution. Results of the Canonical Correspond-ence Analysis (CCA) explained 76.8 % of the correlation between species and environmental variables. Salinity was the most effective variable on species. The highest functional divergence and functional richness were found in the elevational ranges of 800 - 899 m and 1100 -1199 m, respectively. When the highest functional evenness was accounted for the 1400 -1499 m, the functional dispersion was found higher in the range of 1200 -1299 m. A medium length of the carapace, the left valve overlaps the right valve, long swimming setae on the second antenna, a well-developed uropod, and a smooth carapace surface are the common traits of the species used in FD. Sampling sites were clustered by the common species, which also showed correlation with four cations (Ca, Na, Mg, K). Results suggest that 'functional diversity' can be used to correlate ostracod traits with their functional ecology.Öğe Prevalence of Nosemosis and Varroosis in honey bees in Sinop province(Kastamanu University, 2023) Yaman, Mustafa; Güvendik, Tuğba Sağlam; Ünal, SabriAbstract Aim of study: Sinop province has a potential to progress in beekeeping. In this study, it was aimed to investigate the presence and distribution of nosemosis and varroosis in honey bees in Sinop province. Area of study: 12 villages from 7 districts (Ayancık, Boyabat, Dikmen, Durağan, Erfelek, Gerze and Saraydüzü) in Sinop province were determined as study area. Material and methods: A total of 874 adult bee samples were dissected in Ringer’s solution and examined for nosemosis. A total of 1.640 adult bee samples were examined for varroosis. Main results: Nosemosis was the most common disease. Average of the nosemosis infection was 7.6%. On the other hand, varroosis was observed in the eight of 12 localities examined in Sinop province. Total infestation ranged from 0.6 to 8.2%. Highlights: Nosemosis and varroosis were observed in eight (66.7%) of the 12 examined localities in Sinop province. These data are considered as proof that honey production is negatively affected. This study also confirms that the beekeepers in the Sinop province should be informed in detail about the precautions to be taken in the monitoring and controlling varroosis and nosemosis.Öğe Revealing genetic diversity of tulips in Turkey with inter-simple sequence repeat markers(Springer, 2023) Hacıoğlu, Burcu Tarıkahya; Eker, İsmailIn order to reveal the genetic variation within the genus Tulipa, 57 accessions belonging to 19 taxa that are native to Turkey were examined in our study. With inter-simple sequence repeat markers, a matrix of 4332 data was obtained with 76 polymorphic bands. The unweighted pair group average (UPGMA) clustering algorithm was used for the Cluster Analysis. According to dendrogram, two main groups were formed: First group comprised of Tulipa sylvestris subsp. australis, Tulipa sprengeri, Tulipa humilis, Tulipa koyuncui, Tulipa undulatifolia var. undulatifolia and Tulipa julia accessions. Except Tulipa undulatifolia var. undulatifolia and Tulipa julia members of the first group belong to subgenus Eriostemones. In the subclades of the second main group the members of subgenus Tulipa and the members of subgenus Eriostemones were grouped in accordance to their subgenera. Our study is the first attempt to reveal the genetic diversity with all geographical distribution areas of Tulipa in Turkey. We think that the data given here will be useful for both plant taxonomists and ornamental plant breeders for future research.Öğe Fritillaria ecerii (Liliaceae), a new species from Southeastern Anatolia, Turkey(Finnish Zoological and Botanical Publishing Board, 2023) Eker, İsmail; Balos, Mehmet MarufFritillaria ecerii Balos & Eker sp. nova (Liliaceae) is described from the province of Mardin, SE Anatolia, Turkey. It is morphologically similar to F. melananthera in having a striped perigone, to F. caucasica and F. baskilensis in having a long style, and to F. assyriaca in having the same number of leaves. However, it clearly differs from F. melananthera and F. assyriaca mainly by its smooth style, longer filaments and more numerous bracts, and from F. caucasica and F. baskilensis mainly by its striped perigone and more numerous bracts. Diagnostic characteristics, a description, images, and a conservation assessment are provided.Öğe Comparative de novo transcriptome analysis and random UV mutagenesis: Application in high biomass and astaxanthin production enhancement for Haematococcus pluvialis(Springer, 2023) Telli, Murat; Ünlü, Ercan SelçukBackgroundAstaxanthin is a natural carotenoid with strong antioxidant capacity. The high demand on astaxanthin by cosmetic, food, pharmaceutical and aquaculture industries promote its value in the biotechnological research. Haematococcus pluvialis Flotow 1844 has been characterized as one of the most promising species for natural astaxanthin biosynthesis. Even though H. pluvialis as an advantage in producing astaxanthin, its slow grow-yield limits usage of the species for large-scale production.Methods and ResultsIn this study we generated mutated H. pluvialis strain by using one-step random UV mutagenesis approach for higher biomass production in the green flagellated period and in turn higher astaxanthin accumulation in red stage per unit algae harvest. Isolated mutant strains were tested for the astaxanthin accumulation and yield of biomass. Among tested strains only mutant strain designated as only MT-3-7-2 showed a consistent and higher growth pattern, the rest had shown a fluctuated and then decreased growth rate than wild type. To demonstrate the phenotypical changes in MT-3-7-2 is associated with transcriptome, we carried out comparative analysis of transcriptome profiles between MT-3-7-2 and the wild type strains. De novo assembly was carried out to obtain the transcripts. Differential expression levels for the transcripts were evaluated by functional annotation analysis.ConclusionsData showed that increased biomass for the MT-3-7-2 strain was different from wild type with expression of transcripts upregulated in carbohydrate metabolism and downregulated in lipid metabolisms. Our data suggests a switching mechanism is enrolled between carbohydrate and lipid metabolism to regulate cell proliferation and stress responses.Öğe A new ostracod species of the genus Xestoleberis from Nansen Island, Wilhelmina Bay, Southern Ocean, Antarctica(Springer, 2023) Külköylüoğlu, OkanComparing to the ostracod studies on the other islands in the world, studies on the ostracod fauna of the islands in Antarctica are scarce. During the sixth Turkish Antarctica Expedition (TAE 6) to the islands (Dismal, Horseshoe, Nansen, Livingstone) in Western Antarctica, sediment and water samples were collected from 32 different water bodies (lakes, ponds, creeks, springs, littoral zones of sea). Among several new reports of different taxonomic groups which are under investigation, a new marine ostracod species (Xestoleberis nansenensis n. sp.) was encountered from Nansen Island. This is the first report of an ostracod from this island below 60 & DEG; S in Antarctica. The new species has several different features both in the carapace and soft body parts. Absence of ostracods from other islands sampled may be related to several a/biotic factors, such as water chemistry (e.g., relatively low calcium levels), extreme aquatic conditions (e.g., low-temperature values), improper habitat conditions (e.g., too little or absence of sediment in the water bodies), and isolation of the island(s) from the mainland. Although our new species is a marine form, comparative literature review indicated that there is no non-marine living ostracod species reported from the islands visited during the expedition. Possible reasons are discussed.Öğe Meta-analysis of sugar beet (Beta vulgaris L.) transcriptome profiles under different biotic and abiotic stress conditions(Springer, 2023) Bulut, Burak; Gürel, Songül; Ünüvar, Ömer Can; Gürel, Ekrem; Ünlü, Ercan SelçukSugar beet (Beta vulgaris L.) meets the 21% of world sugar production. Soil pollution, biotic and abiotic factors in production areas greatly reduce product quantity and quality. Sugar beet responds to biotic and abiotic stresses such as drought, salt, heat, light, and infections of nematode, bacteria and fungi at the molecular level. Understanding molecular mechanisms require comprehensive genomics studies in order to control these mechanisms to increase the yield and quality. Transcriptome studies performed under stress conditions can shed light on the responses of plants at the molecular level. In addition, meta-analysis can help to find common responses under different stress conditions. In this study four different stress-related transcriptome data were used: two of them are related with biotic stress (nematode and fungi infection) and two of them are related with abiotic stress (ABA treatment and salt stress). In this study, we performed meta-analysis of studies conducted under biotic and abiotic stress conditions. Our results revealed 460 commonly regulated genes from biotic stress related data and 1031 commonly regulated genes from abiotic stress related data. Our data also showed that expression of ten genes is controlled regardless of the type of stress condition. The data can be useful for understanding the molecular aspect of adaptive stress response in sugar beet.Öğe Allium mehmetyaschari (Amaryllidaceae), a new species from southern Anatolia, Turkey(Finnish Zoological and Botanical Publishing Board, 2023) Eker, İsmailAllium mehmetyaschari Eker sp. nova (Amaryllidaceae) is described as a new species from the Province of Adana, southern Anatolia, Turkey. It is morphologically similar to A. sintenisii and A. erzincanicum in having bearded outer tepals. However, it clearly differs from A. sintenisii by having e.g., smaller bulb, stem and leaves; fewer bulblets; different sheath indumentum; different shape and size of umbel; shorter pedicels; smaller flowers and reproductive organs; different perigone colour; different indumentum structure on outer tepals; smooth inner tepals; and smaller capsule and seeds. It differs from A. erzincanicum mainly in tunic type; leaf structure; densely scabrid sheaths; umbel shape; indumentum shape on outer tepals; smooth inner tepals; ratio of central cup to lateral cups; anther colour; and in having bulblets. Herein, the morphological features, a comprehensive description, habitat and ecological characteristics, detailed images, conservation assessment and micromorphological characteristics of the seeds of A. mehmetyaschari are provided.Öğe Neuroprotective effects of goji berry (Lycium barbarum L.) polysaccharides on depression-like behavior in ovariectomized rats: Behavioral and biochemical evidence(Medicinska Naklada, 2023) Soytürk, Hayriye; Bozat, Bihter Gökçe; Karakaş, Fatma Pehlivan; Coşkun, Hamit; Fırat, TülinAim To assess the protective effects of goji berry (Lycium barbarum L.) polysaccharides (LBP) on depression-like behavior in ovariectomized rats and to elucidate the mechanisms underlying these effects. Methods One hundred female Wistar albino rats (three months old) were randomly assigned either to ovariectomy (n = 50) or sham surgery (n = 50). After a 14-day recovery period, the groups were divided into five treatment subgroups (10 per group): high-dose LBP (200 mg/kg), low-dose LBP (20 mg/kg), imipramine (IMP, 2.5 mg/kg), 17 beta estradiol (E2, 1 mg/kg), and distilled water. Then, rats underwent a forced swimming test. We also determined the levels of serum antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde), E2 levels, hippocampal brain-derived neurotrophic factor (BDNF), 5HT2A receptor, and transferase dUTP nick end labeling (TUNEL)-positive cells. Results Both low-dose LBP and imipramine decreased depression-like behavior by increasing serum superoxide dismutase activity and by decreasing serum malondialdehyde level. Furthermore, low-dose LPB, high-dose LBP, and imipramine increased the number of 5-HT2A receptor-and BDNF-positive cells but decreased the number of TUNELpositive cells in the hippocampus. Conclusion This is the first study to show the antidepressant effect of LBP. Although additional research is needed, LBP may be considered a potential new antidepressant.Öğe Do vermicompost applications ımprove growth performance, pharmaceutically ımportant alkaloids, phenolic content, free radical scavenging potency and defense enzyme activities in summer snowflake (Leucojum aestivum L.)?(Wiley-VCH Verlag GMBH, 2023) Çimen, Ayça; Baba, Yavuz; Yıldırım, Arzu Birinci; Türker, Arzu UçarLeucojum aestivum L. contains galanthamine and lycorine, which are two pharmaceutically valuable alkaloids. Vermicompost (VC), an organic waste product created by earthworms enhances soil quality and can improve the medicinal quality of the plant that is crucial to the pharmaceutical industry. The aim of this study was to determine the effects of four different VC concentrations (5 %, 10 %, 25 %, and 50 %) on L. aestivum growth parameters, alkaloid levels (galanthamine and lycorine), total phenol-flavonoid content, free radical scavenging potential, and defense enzyme activities (SOD and CAT) compared to control (no VC). The width, length, and fresh weight of the leaves were improved by 10 % VC treatment. The highest total phenolic content was found in the bulbs and leaves treated with 50 % VC. HPLC-DAD analysis of alkaloids showed that 10 % and 50 % VC treatments contained the most galanthamine in the bulb and leaf extracts, respectively. The application of 25 % VC was the most efficient in terms of lycorine content in both extracts. CAT activity was elevated at 10 %, 25 %, and 50 % VC. Based on the growth performance and galanthamine content of the bulbs and leaves, it can be concluded that a 10 % VC application was the most effective in the cultivation of L. aestivum.Öğe The effect of carotid artery cannulation and femoral artery cannulation with the cardiac vagal denervation on the ischemia and reperfusion induced arrhythmia(Wiley, 2023) Özdemir, Şevval; Bozdoğan, ÖmerBaroreceptor Myocardial Ischemia; Reperfusion; Arrhythmia; Vagal; Denervation