The effect of astaxanthine on ischemia-reperfusion injury in a rat model
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Dosyalar
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elseiver
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: We aimed to compare biochemical and histopathological findings of astaxanthin's potential effects on oxidative stress in ischemia/reperfusion damage (I/R). Methods: Thirty-two rats were randomly divided into four groups: control group; I/R group; I/ R + treatment group; drug group. Astaxanthin was orally administered to groups C and D for 14 days. In groups B and C, the femoral artery was clamped for 2 h to form ischemia. The clamp was opened, and reperfusion was performed for 1 h. In all groups, 4 ml of blood sample through intracardiac puncture and gastrocnemius muscle tissue samples were collected. Serum and tissue samples were analyzed by measuring malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (TAC), and total oxidative level (TOL). Necrosis, inflammation, and caspase-3 in muscle tissue collected for histopathological examination were evaluated. Results: Tissue MDA, SOD and TOL values significantly differed between groups. Serum MDA, SOD, TOL and TAC values significantly differed between groups. On necrosis examination, there was a significant difference between groups B and C. Although signs of inflammation significantly differed between groups, there was no significant difference between groups A and C and groups A and D. Although there was a significant difference in caspase-3 results between groups, there was no significant difference between groups A and C. Conclusions: The use of astaxanthin before and after surgery showed preventive or therapeutic effects against I/R damage. (C) 2021 The Japanese Orthopaedic Association. Published by Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Astaxanthin, Ischemia/Reperfusion, Oxidative Stress, Skeletal-Muscle, Damage, Genotoxicity
Kaynak
Journal of Orthopaedic Science
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
27
Sayı
5
Künye
Durukan, Y., Bala, M. M., Şahin, A. A., Fırat, T., Buğdaycı, G., & Özturan, K. E. (2022). The effect of astaxanthine on ischemia-reperfusion injury in a rat model. Journal of Orthopaedic Science, 27(5), 1132-1138.
