Montelukast protects axial pattern rat skin flaps against ischemia/reperfusion injury
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Dosyalar
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Inc Elsevier Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background. Recent studies have shown that neutrophils play an important role in the pathogenesis of reperfusion injury. Using an inferior epigastric artery skin flap as a flap ischemia/reperfusion (I/R) injury model, we investigated whether the administration of montelukast sodium, a selective reversible cysteinyl leukotriene 1 (Cy5LT1) receptor antagonist, decreases neutrophil infiltration and promotes flap survival. Methods. Eighteen rats were used and randomly divided into three groups (n = 6 for each group). Group I was the sham group and did not undergo ischemic insult; rather, normal saline (1mL) was administrated intraperitonealy (i.p.) 30 min before surgery and continued for 6 d. Group II (control) and Group III (montelukast) underwent 12 h of ischemic insult. For Group II, normal saline (1mL) was injected i.p. 30 min before the surgery and immediately before reperfusion, and this continued for 6 d. In Group III, 1mL of montelukast (10mg/kg) was injected i.p. and continued for 6 d. Malondialdehyde (MDA) and glutathione (GSH) levels and myeloperoxidase (MPO) enzyme activities were investigated. Histological evaluation was made to investigate the tissue neutrophil count. Survival areas were assessed at 7 d postoperatively. Results. Group III (montelukast- treated) showed a significantly higher survival rate than Group II (control) (P = 0.029) but a lower survival rate than Group I (sham). Histological and biochemical assays corroborated this data. Conclusion. This study suggests that montelukast CysLT1 receptor antagonist montelukast reversed I/R-induced oxidant responses and improved flap survival by inhibiting neutrophil infiltration and balancing oxidant and antioxidant status. (C) 2009 Elsevier Inc. All rights reserved.
Açıklama
Anahtar Kelimeler
Montelukast, Ischemia Reperfusion, Injury, Axial Pattern Skin Flap
Kaynak
Journal Of Surgical Research
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
157
Sayı
2
