In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress
Yükleniyor...
Dosyalar
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Mature embryos of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat were used for
callus induction on media containing four diferent doses (0, 1, 2 and 4 mg L−1) of 2,4-D and dicamba supplemented with
fve diferent boron concentrations (0, 6.2, 12.4, 24.8, and 37.2 mg L−1). The obtained callus was transferred to culture media
with three (0, 0.5, and 2 mg L−1) diferent BAP doses with fve boron concentrations for further regeneration. The maximum
callus weight in einkorn wheat was in culture media with 1 mg L−1 dicamba and 6.2 mg L−1 (3.71±0.13 g). Bread wheat had
the maximum callus weight on culture media with 4 mg L−1 dicamba and 12.4 mg L−1 (3.46±0.40 g). The highest plantlet
numbers were in only 2 mg L−1 BAP (2.92±0.88) for einkorn wheat and 0.5 mg L−1 BAP supplemented with 6.2 mg L−1
boron (3.71±1.12) for bread wheat. This indirect regeneration protocol using mature embryos of einkorn and bread wheat
under boron stresses expected to be useful for future wheat breeding studies.
Açıklama
We would like to thank Bolu Abant Izzet Baysal University, Scientific Research Projects Fund (BAP - 2018.03.01.1296) for their financial contribution to this research.
Anahtar Kelimeler
Boron Stress, Bread, Callus, Einkorn, Mature Embry
Kaynak
Plant Cell Tissue and Organ Culture
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
148
Sayı
2
Künye
Ağıl, F., Örgeç, M., Karakaş, F. P., Verma, S. K., & Zencirci, N. (2021). In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress. Plant Cell, Tissue and Organ Culture (PCTOC), 1-12.
