Protective effect of Coriandrum sativum extract against inflammation and apoptosis in liver ischaemia/reperfusion injury

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dc.authorid0000-0002-8733-2096en_US
dc.contributor.authorKükner, Aysel
dc.contributor.authorSöyler, Gizem
dc.contributor.authorToros, Pelin
dc.contributor.authorDede, Gizem
dc.contributor.authorMeriçli, Filiz
dc.date.accessioned2023-07-11T12:23:54Z
dc.date.available2023-07-11T12:23:54Z
dc.date.issued2021en_US
dc.departmentBAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground: The aim of this study was to investigate the anti-inflammatory and antioxidant effects of Coriandrum sativum extract on liver ischaemia reperfusion injury at light microscopic and biochemical levels. Materials and methods: Sham, ischaemia/reperfusion injury (IRI), IRI + Coriandrum sativum extract and only Coriandrum sativum extract groups were formed. Sixty minutes of ischaemia and 60 minutes of reperfusion were performed. In the treatment group, 300 mg/kg/day Coriandrum sativum was given by gavage. Hepatic tissues were fixed in 4% paraformaldehyde. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) enzymes were measured. Nuclear factor-kappa beta (NF-kappa B), tumour necrosis factor-alpha (TNF-alpha) and caspase-3 immunohistochemistry staining was performed. Microscopic scoring was performed in terms of sinusoidal congestion, vacuolisation, and necrosis. Results: Sinusoidal enlargement and diffuse congestion, Kupffer cell increase, neutrophil increase in necrotic areas, vacuolisation in hepatocytes, and bile duct proliferation in the portal triad were observed in ischaemia/reperfusion hepatic tissue. Very rare, necrotic areas were observed in the Coriandrum sativum treatment group, while congestion and vacuolisation and bile duct proliferation were decreased compared to the ischaemic group. The AST and ALT levels were increased in the IRI and IRI + Coriandrum sativum groups. When compared to the IRI group, the AST and ALT levels of the Coriandrum sativum were considerably decreased. The IRI and IRI + Coriandrum sativum groups had statistically significant differences in ALP compared to that of the Coriandrum sativum and Sham groups. There was no significant difference between the ALP levels of the IRI and IRI + Coriandrum sativum groups TNF-alpha, NF-kappa B and caspase-3 immune positive stained hepatocytes were numerous and widely observed in the injury group. There were positive TNF-alpha immunohistochemical staining Kupffer cells in the IRI group. In the group treated with Coriandrum sativum, Kupffer cells were not stained, while TNF-alpha, NF-kappa B and caspase-3 expressing hepatocytes were found to be decreased compared to the IRI group. When the expression values of the TNF-alpha, NF-kappa B and caspase-3 groups were evaluated statistically, it was seen that there was a significant decrease in the group treated with Coriandrum sativum. Conclusions: It was found that Coriandrum sativum extract decreased proinflammatory cytokine TNF-alpha and apoptotic cell death and liver enzymes in liver ischaemia/reperfusion injury.en_US
dc.identifier.citationKükner, A., Soyler, G., Toros, P., Dede, G., Meriçli, F., Işık, S., ... & Özoğul, C. (2021). Protective effect of Coriandrum sativum extract against inflammation and apoptosis in liver ischaemia/reperfusion injury. Folia Morphologica, 80(2), 363-371.en_US
dc.identifier.doi10.5603/FM.a2020.0060
dc.identifier.endpage371en_US
dc.identifier.issn0015-5659
dc.identifier.issn1644-3284
dc.identifier.issue2en_US
dc.identifier.pmid32488856en_US
dc.identifier.scopus2-s2.0-85107903135en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage363en_US
dc.identifier.urihttp://dx.doi.org/10.5603/FM.a2020.0060
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11270
dc.identifier.volume80en_US
dc.identifier.wosWOS:000661271600018en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorDede, Gizem
dc.language.isoenen_US
dc.publisherVIA MEDICAen_US
dc.relation.ispartofFolia Morphologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLiver Ischaemiaen_US
dc.subjectCoriandrum Sativumen_US
dc.subjectNuclear Factor-Kappa Betaen_US
dc.subjectTumour Necrosis Factor-Alphaen_US
dc.subjectCaspase-3en_US
dc.subjectIschemia-Reperfusion Injuryen_US
dc.titleProtective effect of Coriandrum sativum extract against inflammation and apoptosis in liver ischaemia/reperfusion injuryen_US
dc.typeArticleen_US

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