Transcription of the HXT4 gene is regulated by Gcr1p and Gcr2p in the yeast S-cerevisiae

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Küçük Resim

Tarih

1999

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

John Wiley & Sons Ltd

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Glucose transport and glycolysis are two sequential events which are regulated by both physiological and environmental signals in the yeast Saccharomyces cerevisiae. Transcription of the HXT4 gene was found to be regulated by Gcr1p and Gcr2p, transcription factors that are required for the regulated high level transcriptions of glycolytic genes. Transcription of HXT4 decreased about 35-fold in gcr1 mutant and two-fold in gcr2 mutant yeast cells. However, transcription of other HXT genes was not affected at a significant level by gcr1 or gcr2 mutations. Overproduction of Gcr1p from an inducible promoter resulted in a 15-64% increase in transcription of HXT4, depending on the growth conditions. Gel mobility shift assays performed with the purified DNA binding domain of Gcr1p and the UAS region of the HXT4 gene showed that Gcr1p interacts directly with multiple sites on the HXT4 UAS region. These results indicate that Gcr1p and Gcr2p coordinate the transcription of HXT4 and glycolytic genes.

Açıklama

Anahtar Kelimeler

Yeast, Hexose Transporters, Gcr1p, Transcriptional Regulation

Kaynak

Yeast

WoS Q Değeri

Q1

Scopus Q Değeri

Q2

Cilt

15

Sayı

11

Künye