Effect of hypericin on the ADAMTS-9 and ADAMTS-8 gene expression in MCF7 breast cancer cells

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dc.authorid0000-0003-4357-5229en_US
dc.contributor.authorOcak, Zeynep
dc.contributor.authorAcar, Muradiye
dc.contributor.authorGündüz, E.
dc.contributor.authorGündüz, Mehmet
dc.contributor.authorDemircan, Kadir
dc.contributor.authorÜyeturk, Ümmügül
dc.contributor.authorÖzlü, Tülay
dc.date.accessioned2021-06-23T19:34:35Z
dc.date.available2021-06-23T19:34:35Z
dc.date.issued2013
dc.departmentBAİBÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractAIM: To investigate the effects of hypericin which is obtained from the plant Hypericum perforatum on the expression and the regulation of ADAMTS8 and ADAMTS9 genes in MCF7 breast cancer cells and on the viability of these cells. MATERIALS AND METHODS: MCF7 cells were cultured and were separately exposed to 2, 10 and 50 mu l/mL of hypericin. After 24 hours, RNA was isolated from these cells and converted to cDNA. The expression levels of ADAMTS8 and ADAMTS9 genes were evaluated using the Reverse Transcription Polymerase Chain Reaction. XTT (2,3-bis-( 2-methoxy-4-nitro-5-sulfophenyl)-2Htetrazolium-5-carboxanilide, disodium salt) cell viability assay was used to determine cytotoxicity. RESULTS: ADAMTS9 expression in MCF7 cells were increased 1.8 and 3.6 fold with the use of 2 and 10 mu l/mL of hypericin, respectively; and decreased 0.7 fold with the use of 50 mu l/mL of hypericin. There was no significant change in the ADAMTS8 expression. Rapid cell death was observed in the cancer cells when hypericin was used at a dose of >= 50 mu l/mL. CONCLUSIONS: The increase in ADAMTS9 expression can be a useful factor in the prevention of possible metastasis in breast cancer and for the occurrence of a tumor suppressive effect. Hypericin increases the expression of ADAMTS9, therefore, it may show its antitumoral and anti-apoptotic effects by means of ADAMTS9.en_US
dc.identifier.endpage1190en_US
dc.identifier.issn1128-3602
dc.identifier.issue9en_US
dc.identifier.pmid23690187en_US
dc.identifier.scopus2-s2.0-84878526002en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1185en_US
dc.identifier.urihttps://www.europeanreview.org/wp/wp-content/uploads/1185-1190.pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7563
dc.identifier.volume17en_US
dc.identifier.wosWOS:000319518300007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorOcak, Zeynep
dc.institutionauthorÜyeturk, Ümmügül
dc.institutionauthorÖzlü, Tülay
dc.language.isoenen_US
dc.publisherVerduci Publisheren_US
dc.relation.ispartofEuropean Review For Medical And Pharmacological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectADAMTS9en_US
dc.subjectADAMTS8en_US
dc.subjectHypericinen_US
dc.subjectBreast canceren_US
dc.titleEffect of hypericin on the ADAMTS-9 and ADAMTS-8 gene expression in MCF7 breast cancer cellsen_US
dc.typeArticleen_US

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