Silibinin effect on methotrexate-induced hepatotoxicity in rats
Yükleniyor...
Dosyalar
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Aves
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: Hepatotoxicity is one of the major side effects of methotrexate and limits its use. In this study, we investigated the hepatoprotective effect of silibinin and the role of oxidative stress markers and cytokines on high-dose methotrexate-induced hepatotoxicity in rats.
Materials and Methods: In this study, rats were randomly divided into 5 groups (n = 7). Methotrexate (20 mg/kg, intraperitoneally) was administered on the first day in all groups except control. Silibinin was injected for 5 days to methotrexate-silibinin25, methotrexate-silibinin50, and methotrexate-silibinin 100 groups at a dose of 25, 50, arid 100 mg/kg/day, respectively. On the sixth day. blood and liver samples were obtained and rats were sacrificed. Serum total antioxidant capacity, total oxidant status, total thiol, native thiol, alanine aminotransferase, aspartate transaminase, bilirubin, albumin, tumor necrosis factor-alpha, and interleukin-10 levels were measured. In addition, a histopathological evaluation of liver tissues was performed.
Results: Methotrexate reduced total antioxidant capacity and increased disulfide/total thiol ratio. Histopathologic examination revealed that methotrexate increased hepatic damage and 50 mg/kg/dose of silibinin prevented inflammatory cell infiltration in particular.
Conclusion: Our results suggest that silibinin (50 mg/kg/day) may reduce the hepatic damage in rnethotrexate-induced hepatotoxicity in rats by increasing antioxidant capacity.
Açıklama
Sakarya University Scientific Research Project Unit
Anahtar Kelimeler
Methotrexate, Hepatotoxicity, Silibinin, Oxidative Stress, Thiol/Disulfide, Milk Thistle
Kaynak
Eurasian Journal of Medicine
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
54
Sayı
3
Künye
Yanaşoğlu, E., Büyükavcı, M., Çetinkaya, A., Turan, G., Köroğlu, M., Yazar, H., & Büyükokuroğlu, M. E. (2022). Silibinin Effect on Methotrexate-Induced Hepatotoxicity in Rats. The Eurasian Journal of Medicine, 54(3), 264.
