Efficient doxorubicin loading to isolated dexosomes of immature JAWSII cells: Formulated and characterized as the bionanomaterial

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dc.authorid0000-0003-1956-4474en_US
dc.authorid0000-0002-8212-7149en_US
dc.authorid0000-0001-9199-4087en_US
dc.authorid0000-0003-3682-2068en_US
dc.authorid0000-0002-3179-6342
dc.authorid0000-0001-7712-2813
dc.authorid0000-0002-7376-3709
dc.contributor.authorMutlu, Esra Cansever
dc.contributor.authorKaya, Özge
dc.contributor.authorWood, Matthew
dc.contributor.authorMager, Imre
dc.contributor.authorTopkara, Kübra Çelik
dc.contributor.authorÇamsarı, Çağrı
dc.contributor.authorYıldırım, Arzu Birinci
dc.contributor.authorÇetinkaya, Ayhan
dc.date.accessioned2021-06-23T19:54:01Z
dc.date.available2021-06-23T19:54:01Z
dc.date.issued2020
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractImmature dendritic cells (IDc), 'dexosomes', are promising natural nanomaterials for cancer diagnose and therapy. Dexosomes were isolated purely from small-scale-up production by using t25-cell-culture flasks. Total RNA was measured as 1.43 +/- 0.33 ng/10 cell. Despite the fact that they possessed a surface that is highly abundant in protein, this did not become a significant effect on the DOX loading amount. Ultrasonication was used for doxorubicin (DOX) loading into the IDc dexosomes. In accordance with the literature, three candidate DOX formulations were designed as IC50 values; dExoIII, 1.8 mu g/mL, dExoII, 1.2 mu g/mL, and dExoI, 0.6 mu g/mL, respectively. Formulations were evaluated by MTT test against highly metastatic A549 (CCL-185; ATTC) cell line. Confocal images of unloaded (naive) were obtained by CellMask(TM)membrane staining before DOX loading. Although, dexosome membranes were highly durable subsequent to ultrasonication, it was observed that dexosomes could not be stable above 70 degrees C during the SEM-image analyses. dExoIII displayed sustained release profile. It was found that dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) results were in good agreement with each other. Zeta potentials of loaded dexosomes have approximately between -15 to -20 mV; and, their sizes are 150 nm even after ultrasonication. IDcJAWSII dexosomes can be able to be utilized as the "BioNanoMaterial" after DOX loading via ultrasonication technique.en_US
dc.identifier.doi10.3390/ma13153344
dc.identifier.issn1996-1944
dc.identifier.issue15en_US
dc.identifier.pmid32727156en_US
dc.identifier.scopus2-s2.0-85089747076en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.3390/ma13153344
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10380
dc.identifier.volume13en_US
dc.identifier.wosWOS:000559122300001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaya, Özge
dc.institutionauthorÇamsarı, Çağrı
dc.institutionauthorÇetinkaya, Ayhan
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofMaterialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectJAWSIIen_US
dc.subjectExosomeen_US
dc.subjectDoxorubicinen_US
dc.subjectUltrasonicationen_US
dc.subjectA549 Cellsen_US
dc.subjectNatural Nanoparticlesen_US
dc.titleEfficient doxorubicin loading to isolated dexosomes of immature JAWSII cells: Formulated and characterized as the bionanomaterialen_US
dc.typeArticleen_US

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