In vitro propagation and cardenolide profiling of Digitalis ferruginea subsp schischkinii, a medicinally important foxglove species with limited distribution in Northern Turkey

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dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0002-7631-823Xen_US
dc.authorid0000-0001-6767-2539en_US
dc.authorid0000-0002-6786-3973
dc.authorid0000-0002-5647-7559
dc.authorid0000-0002-7376-3709
dc.contributor.authorYücesan, Buhara
dc.contributor.authorMohammed, Aliyu
dc.contributor.authorEker, İsmail
dc.contributor.authorSameeullah, Muhammad
dc.contributor.authorDemir-Ordu, Öznur
dc.contributor.authorCihangir, Cansu
dc.contributor.authorŞahbaz, Nevin
dc.contributor.authorKaya, Özge
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:43:32Z
dc.date.available2021-06-23T19:43:32Z
dc.date.issued2016
dc.departmentBAİBÜ, Ziraat Fakültesi, Bahçe Bitkileri Bölümüen_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Tohum Bilimi ve Teknolojisi Bölümü
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractCardenolides isolated from genus Digitalis are natural compounds used in treating cardiac insufficiencies. Recent investigations have also suggested the use of cardenolides in oncology. Due to the medicinal importance of the genus Digitalis, in vitro regeneration techniques would be useful for propagating the best genotypes selected from natural sources. To achieve this, Digitalis ferruginea subsp. schischkinii (Ivanina) K. Werner was collected from different locations along the Black Sea region of Turkey. In the cardenolide analysis, samples from Giresun and Trabzon showed a greater diversity than those from other locations, and thus, the Trabzon population was selected for plant tissue culture studies. In vitro regeneration was performed using cotyledonary leaves and hypocotyl segments on Murashige and Skoog (MS) medium containing various concentrations (0.5, 1.0, or 2.0 mg L-1) of 6-benzyladenine (BA), kinetin (KIN), or thidiazuron (TDZ) alone or in combination with indole-3-acetic acid (IAA) at 0.5 or 1.0 mg L-1. Among the media used for shoot regeneration, the highest frequency of shoot organogenesis was obtained from MS medium containing 2.0 mg L-1 TDZ and 0.5 mg L-1 IAA, producing 11.0 shoots per explant with 100% regeneration frequency from hypocotyl explants after 4 wk. For successful rooting, IAA was the most effective at 1.0 or 2.0 mg L-1, producing 16.3 or 17.3 roots per shoot, respectively, after 4 wk. Regenerants from several medium types were grown in a greenhouse for 6 mo, and all displayed similar cardenolide contents, i.e., the lanatoside C level was around 0.3% (w/w) while the levels of lanatosides A and B, digoxin, and digitoxin were less than 0.08% (w/w), similar to plants grown from seeds collected from Trabzon district.en_US
dc.identifier.doi10.1007/s11627-016-9759-4
dc.identifier.endpage329en_US
dc.identifier.issn1054-5476
dc.identifier.issn1475-2689
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84964555331en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage322en_US
dc.identifier.urihttps://doi.org/10.1007/s11627-016-9759-4
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8798
dc.identifier.volume52en_US
dc.identifier.wosWOS:000379267000012en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorYücesan, Buhara
dc.institutionauthorMohammed, Aliyu
dc.institutionauthorEker, İsmail
dc.institutionauthorSameeullah, Muhammad
dc.institutionauthorDemir-Ordu, Öznur
dc.institutionauthorCihangir, Cansu
dc.institutionauthorŞahbaz, Nevin
dc.institutionauthorKaya, Özge
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-Planten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCardenolidesen_US
dc.subjectDigitalis Ferruginea Subspen_US
dc.subjectSchischkiniien_US
dc.subjectFoxgloveen_US
dc.subjectIn Vitro Cultureen_US
dc.titleIn vitro propagation and cardenolide profiling of Digitalis ferruginea subsp schischkinii, a medicinally important foxglove species with limited distribution in Northern Turkeyen_US
dc.typeArticleen_US

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