An efficient regeneration system and steviol glycoside analysis of Stevia rebaudiana Bertoni, a source of natural high-intensity sweetener

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dc.authorid0000-0003-2379-5363en_US
dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0001-6767-2539en_US
dc.authorid0000-0002-6786-3973
dc.authorid0000-0003-4740-1138
dc.contributor.authorYücesan, Buhara
dc.contributor.authorBüyükgöçmen, Refik
dc.contributor.authorMohammed, Aliyu
dc.contributor.authorSameeullah, Muhammad
dc.contributor.authorAltuğ, Cevher
dc.contributor.authorGürel, Songül
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:43:29Z
dc.date.available2021-06-23T19:43:29Z
dc.date.issued2016
dc.departmentBAİBÜ, Ziraat Fakültesi, Tohum Bilimi ve Teknolojisi Bölümüen_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Bahçe Bitkileri Bölümü
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractStevia is a natural, zero-calorie, intensively sweet extracted from the leaves of Stevia rebaudiana. Its sweet taste derives from a group of compounds known as steviol glycosides. In this study, an efficient micropropagation protocol for S. rebaudiana was developed for possible commercial implementation using Murashige and Skoog (MS) basal medium without plant growth regulators for most of the process. Direct shoot formation was achieved after cultivation of nodal segments on MS medium with or without plant growth regulators (benzyl amino purine [BAP], kinetin [KIN]) at various concentrations (0.1, 0.5, 1.0, or 2.0 mg L-1). Although all treatments produced two shoots per explants after 3 wk of culture, high concentrations of KIN and BAP (1.0 or 2.0 mg L-1) induced more callus formation. Rooting was achieved on MS medium containing 0.25 mg L-1 indole-3-acetic acid (IAA), which produced 8.1 roots per shoot after 3 wk of cultivation. After acclimatization of the regenerants in a portable greenhouse for 3 wk, all regenerants and seed-derived seedlings were transferred to field conditions for 16 wk. Steviol glycoside contents (% leaf dry weight) did not differ between leaves collected from regenerants and seed-derived plants. Rebaudioside A content ranged from 4.7 to 5.0% (w/w), while stevioside ranged from 6.4 to 6.9% (w/w). There was no significant difference between the two sampling periods (late vegetative and flowering stages) for the plants grown in the field. In this study, a cost-effective in vitro regeneration protocol was established that enables efficient, large-scale in vitro production of S. rebaudiana for field cultivation.en_US
dc.identifier.doi10.1007/s11627-016-9765-6
dc.identifier.endpage337en_US
dc.identifier.issn1054-5476
dc.identifier.issn1475-2689
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84977070918en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage330en_US
dc.identifier.urihttps://doi.org/10.1007/s11627-016-9765-6
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8786
dc.identifier.volume52en_US
dc.identifier.wosWOS:000379267000013en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGürel, Ekrem
dc.institutionauthorYücesan, Buhara
dc.institutionauthorBüyükgöçmen, Refik
dc.institutionauthorMohammed, Aliyu
dc.institutionauthorSameeullah, Muhammad
dc.institutionauthorAltuğ, Cevher
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-Planten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIn vitro regenerationen_US
dc.subjectIntense sweetenersen_US
dc.subjectRebaudioside Aen_US
dc.subjectStevia rebaudianaen_US
dc.subjectSteviol glycosidesen_US
dc.subjectSteviosideen_US
dc.titleAn efficient regeneration system and steviol glycoside analysis of Stevia rebaudiana Bertoni, a source of natural high-intensity sweeteneren_US
dc.typeArticleen_US

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