An efficient regeneration protocol for in vitro direct organogenesis in einkorn (triticum monococcum l.) wheat

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dc.authorid0000-0003-0060-259Xen_US
dc.authorid0000-0002-9446-7538en_US
dc.authorid0000-0003-3460-7575en_US
dc.contributor.authorŞahin, Günce
dc.contributor.authorÖrgeç, Mehmet
dc.contributor.authorZencirci, Nusret
dc.date.accessioned2023-10-25T11:27:10Z
dc.date.available2023-10-25T11:27:10Z
dc.date.issued2022en_US
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.descriptionThis study was supported by the Bolu Abant Izzet Baysal University Research Foundation (Project No: BAP - 2017.03.01.1130) . The authors thank to the Bolu Municipality (2018/2019) for a research granten_US
dc.description.abstractColeoptile, leaf, and root explants of the einkorn (Triticum monococcum ssp. monococcum) were cultured in vitro to obtain an efficient plant regeneration protocol through direct shoot formation by using different combinations and concentrations of various plant growth regulators. A total of 180 different auxin and cytokinin combinations were tested for regeneration. Shoot formation was not observed with the root and leaf explants. Shoot formation was obtained only from the coleoptile explants, with a mean of 1.20 +/- 0.24 shoots/explant and 86.60% of shoot formation frequency and with a 1.20 +/- 0.53 shoots/explant and 80.00% shoot formation frequency on medium supplemented with 0.5 mg L-1 TDZ and 1 mg L-1 TDZ plus 1 mg L-1 NAA, respectively. The shoots were subcultured on the MS medium containing the most effective hormonal combination concurrently continued to shoot and root formation for 45 days. It is noteworthy that 3.66 +/- 0.66 shoots per explant were induced by MS, which contained 1 mg L-1 TDZ plus 1 mg L-1 NAA and 2.0 mg L-1 KIN plus 0.5 mg L-1 NAA for 45 days. Of the different auxin concentrations tested for rooting, 2.0 mg L-1 IAA was predominant, with the greatest number of roots (12.33 +/- 0.88) produced per regenerated shoot. Finally, these well-developed plantlets were acclimatized with a 100% success rate and were transferred to the ex vitro conditions. A highly efficient regeneration protocol for einkorn wheat was developed using somatic tissue as an explant source for the first time.en_US
dc.description.sponsorshipBolu Abant Izzet Baysal University Research Foundation [BAP - 2017.03.01.1130]; Bolu Municipalityen_US
dc.identifier.citationŞahin, G., Örgeç, M., & Zencirci, N. (2022). An efficient Regeneration Protocol for in vitro Direct Organogenesis in Einkorn (Triticum monococcum L.) Wheat. Journal of Agricultural Sciences, 28(3), 412-422.en_US
dc.identifier.doi10.15832/ankutbd.891812
dc.identifier.endpage422en_US
dc.identifier.issn2148-9297
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85137936581en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage412en_US
dc.identifier.trdizinid1138128en_US
dc.identifier.urihttp://dx.doi.org/10.15832/ankutbd.891812
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11793
dc.identifier.volume28en_US
dc.identifier.wosWOS:000877379000007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorŞahin, Günce
dc.institutionauthorZencirci, Nusret
dc.institutionauthorÖrgeç, Mehmet
dc.language.isoenen_US
dc.publisherGALENOS PUBL HOUSEen_US
dc.relation.ispartofJournal of Agricultural Sciences (Tarım Bilimleri Dergisi)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectColeoptileen_US
dc.subjectDirect Regeneration Protocolen_US
dc.subjectTissue Cultureen_US
dc.subjectEinkorn Wheat (Triticum Monococcum ssp. Monococcum)en_US
dc.titleAn efficient regeneration protocol for in vitro direct organogenesis in einkorn (triticum monococcum l.) wheaten_US
dc.typeArticleen_US

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