An efficient regeneration protocol for in vitro direct organogenesis in einkorn (triticum monococcum l.) wheat
Yükleniyor...
Dosyalar
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
GALENOS PUBL HOUSE
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Coleoptile, leaf, and root explants of the einkorn (Triticum monococcum ssp. monococcum) were cultured in vitro to obtain an efficient plant regeneration protocol through direct shoot formation by using different combinations and concentrations of various plant growth regulators. A total of 180 different auxin and cytokinin combinations were tested for regeneration. Shoot formation was not observed with the root and leaf explants. Shoot formation was obtained only from the coleoptile explants, with a mean of 1.20 +/- 0.24 shoots/explant and 86.60% of shoot formation frequency and with a 1.20 +/- 0.53 shoots/explant and 80.00% shoot formation frequency on medium supplemented with 0.5 mg L-1 TDZ and 1 mg L-1 TDZ plus 1 mg L-1 NAA, respectively. The shoots were subcultured on the MS medium containing the most effective hormonal combination concurrently continued to shoot and root formation for 45 days. It is noteworthy that 3.66 +/- 0.66 shoots per explant were induced by MS, which contained 1 mg L-1 TDZ plus 1 mg L-1 NAA and 2.0 mg L-1 KIN plus 0.5 mg L-1 NAA for 45 days. Of the different auxin concentrations tested for rooting, 2.0 mg L-1 IAA was predominant, with the greatest number of roots (12.33 +/- 0.88) produced per regenerated shoot. Finally, these well-developed plantlets were acclimatized with a 100% success rate and were transferred to the ex vitro conditions. A highly efficient regeneration protocol for einkorn wheat was developed using somatic tissue as an explant source for the first time.
Açıklama
This study was supported by the Bolu Abant Izzet Baysal University Research Foundation (Project No: BAP - 2017.03.01.1130) . The authors thank to the Bolu Municipality (2018/2019) for a research grant
Anahtar Kelimeler
Coleoptile, Direct Regeneration Protocol, Tissue Culture, Einkorn Wheat (Triticum Monococcum ssp. Monococcum)
Kaynak
Journal of Agricultural Sciences (Tarım Bilimleri Dergisi)
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
28
Sayı
3
Künye
Şahin, G., Örgeç, M., & Zencirci, N. (2022). An efficient Regeneration Protocol for in vitro Direct Organogenesis in Einkorn (Triticum monococcum L.) Wheat. Journal of Agricultural Sciences, 28(3), 412-422.
