Reliability of the 34 beta E12, keratin 5/6, p63, bcl-2, and AMACR in the diagnosis of prostate carcinoma

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dc.authorid0000-0003-4096-4261
dc.contributor.authorBoran, Çetin
dc.contributor.authorKandıralı, Engin
dc.contributor.authorYılmaz, Fahri
dc.contributor.authorSerin, Erdinç
dc.contributor.authorAkyol, Mesut
dc.date.accessioned2021-06-23T19:27:34Z
dc.date.available2021-06-23T19:27:34Z
dc.date.issued2011
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractObjective: In this study, we aimed to investigate which basal cell marker should be used with alpha-methylacyl coenzyme A racemase (AMACR) to increase diagnostic accuracy in the diagnosis of prostate carcinoma. Materials and methods: A total of 98 cases of prostate biopsy, comprising 65 cases with prostate adenocarcinoma and 33 cases without adenocarcinoma, were included in this study. Prostate-specific antigen (PSA) serum levels before biopsies were obtained. The number of cores with malignant glands and Gleason scores for each case were determined. Paraffin sections were stained immunohistochemically with 34 beta E12, keratin 5/6, p63, bcl-2, and AMACR. Results: According to staining pattern, extensiveness, and intensity of basal cell markers in benign glands, 34bE12 gave the best results. As negative markers for prostate adenocarcinoma, the best markers were p63 and 34bE12. According to the AUC values in ROC curves for both extensiveness and intensity, the arrangement from the best to the worst was 34bE12, p63, bcl-2, and keratin 5/6. The 34bE12 had the best sensitivity and specificity values (95% and 98%, respectively). Staining extensiveness and intensity of keratin 5/6 in malignant glands, and those of bcl-2 in benign glands had statistically significant positive correlation with serum PSA levels. Even though AMACR is a negative marker for benignity, some of the benign glands also had positive immune reaction with AMACR. However, AMACR positivity was usually focal and weak. Nevertheless, intensively stained subjects were also present. No correlation was present between AMACR and basal cell markers. Conclusions: As a result, we suggest that keratin 5/6 and bcl-2 should not be used to identify benign glands in prostate biopsy since they show high positivity in malignant glands and high negativity in benign glands. 34bE12 should be the first choice as a basal cell marker. p63 can be used together with 34bE12, but it may not give additional diagnostic information. When we evaluated the correlation of basal cell markers, we did not find any complementary staining results among basal cell markers. Our study showed that 34bE12 is the most appropriate negative marker to combine with AMACR as a positive marker for the diagnosis of prostate adenocarcinoma. (C) 2011 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.urolonc.2009.11.013
dc.identifier.endpage623en_US
dc.identifier.issn1078-1439
dc.identifier.issn1873-2496
dc.identifier.issue6en_US
dc.identifier.pmid20189848en_US
dc.identifier.scopus2-s2.0-81055137298en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage614en_US
dc.identifier.urihttps://doi.org/10.1016/j.urolonc.2009.11.013
dc.identifier.urihttps://hdl.handle.net/20.500.12491/6846
dc.identifier.volume29en_US
dc.identifier.wosWOS:000297443300005en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBoran, Çetin
dc.institutionauthorKandıralı, Engin
dc.institutionauthorYılmaz, Fahri
dc.institutionauthorSerin, Erdinç
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofUrologic Oncology-Seminars And Original Investigationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCytokeratinen_US
dc.subjectAlpha-Methylacyl Coenzyme A Racemaseen_US
dc.subjectImmunohistochemistryen_US
dc.titleReliability of the 34 beta E12, keratin 5/6, p63, bcl-2, and AMACR in the diagnosis of prostate carcinomaen_US
dc.typeArticleen_US

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