In vivo evaluation of the genotoxic effects of clomiphene citrate on rat reticulocytes: a micronucleus genotoxicity

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dc.authorid0000-0002-5767-7894en_US
dc.authorid0000-0002-1501-9324
dc.authorid0000-0002-6105-0293
dc.authorid0000-0003-1057-3235
dc.contributor.authorDuran, Bülent
dc.contributor.authorÖzdemir, İsmail
dc.contributor.authorDemirel, Yeltekin
dc.contributor.authorÖzdemir, Öztürk
dc.contributor.authorÇetin, Ali
dc.contributor.authorGüven, Aysel
dc.date.accessioned2021-06-23T19:19:33Z
dc.date.available2021-06-23T19:19:33Z
dc.date.issued2006
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractObjective: To determine the genotoxic effects of clomiphene citrate (CC) on rat reticulocytes in vivo. Methods: In this prospective, randomized, controlled study, rats were each assigned randomly to the CC 50, CC 100, CC 200, or control group and were given repeat doses of 0.16, 0.32 or 0.64 mg CC, or normal saline, respectively. Each study group received its CC dose in 2 ml of saline intraperitoneally for 5 days, while the control group received only 2 ml of saline. Each treatment cycle was repeated six times. Six months later, the rats were euthanized. Bone marrow tissues were removed, and pluripotent reticulocyte cells with micronuclei, nuclear buds, and binuclear abnormalities were analyzed using an in situ micronuclei assay under light microscopy. The proportion of micronucleated erythrocytes was measured. Results: Fewer cells with nuclear buds and binuclear abnormalities were detected in the CC 50 group and controls. The CC 100 and 200 groups had significantly (p < 0.05) more nuclear buds and binuclear abnormalities compared with the CC 50 group and controls in the cytogenetic analysis of bone marrow stem cells. Conclusion: In rats, the micronucleus genotoxicity assay suggests a dose-dependent CC effect on genomic instability in bone marrow stem cells in vivo.en_US
dc.identifier.doi10.1159/000091739
dc.identifier.endpage231en_US
dc.identifier.issn0378-7346
dc.identifier.issn1423-002X
dc.identifier.issue4en_US
dc.identifier.pmid16508321en_US
dc.identifier.scopus2-s2.0-33646728630en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage228en_US
dc.identifier.urihttps://doi.org/10.1159/000091739
dc.identifier.urihttps://hdl.handle.net/20.500.12491/5961
dc.identifier.volume61en_US
dc.identifier.wosWOS:000237698800009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÖzdemir, İsmail
dc.institutionauthorGüven, Aysel
dc.language.isoenen_US
dc.publisherKargeren_US
dc.relation.ispartofGynecologic And Obstetric Investigationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectClomiphene Citrateen_US
dc.subjectInfertilityen_US
dc.subjectMicronucleien_US
dc.subjectRat Modelen_US
dc.titleIn vivo evaluation of the genotoxic effects of clomiphene citrate on rat reticulocytes: a micronucleus genotoxicityen_US
dc.typeArticleen_US

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