The detection of HBV DNA with polymerase chain reaction in blood donors with isolated hepatitis B core antibody
Yükleniyor...
Dosyalar
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Medical Assoc Zenicadoboj Canton
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aim To analyze the presence of HBV DNA in blood donors admitted to blood banks with HBsAg negativity and isolated anti-HBc positivity. Methods Sera samples of 2500 HBsAg negative donors were included in the study. HBsAg tests were assayed with VITROS analyzer. Anti-HBc total, anti-FIBs, HBeAg, anti-HBc and anti-HBc IgM tests were manually studied with DIA PRO kit by employing ELISA method. HBV DNA test was evaluated with iQ5 Real Time PCR Detection System with real-time PCR method. Results A total number of 401 (16.4%) HBsAg negative blood donors had anti-HBc positivity. Forty-five of 401 (1.8%) anti-HBc positive samples were anti-FIBs negative. These 45 persons were evaluated for anti-HBc positivity. Thirty-six of 45 anti-HBc positive persons had only anti-HBc positivity, and other nine patients had Anti-HBe antibody. HBV DNA was detected in three (6.6 %) of 45 isolated Anti-HBc positive persons as in one of nine persons and two of 36 persons. Conclusion The persons who have only FIBs Ag negativity may not be appropriate to become blood donors. Anti-HBc test should be done for HBsAg negative persons. They should not be accepted as blood donors if Anti-HBc is found positive.
Açıklama
Anahtar Kelimeler
Anti-HBc Alone, Blood Banks, DNA
Kaynak
Medicinski Glasnik
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
9
Sayı
2
