Dynamic assessment of Capparis spinosa buds on survival of periodontal ligament cells using a real-time cell analysis method

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dc.authorid0000-0003-4716-948X
dc.authorid0000-0002-5099-5128
dc.authorid0000-0001-9550-1999
dc.contributor.authorOzan, Fatih
dc.contributor.authorOzan, Ülkü
dc.contributor.authorOktay, Elif Aybala
dc.contributor.authorToptaş, Orçun
dc.contributor.authorÖzdemir, Hakan
dc.date.accessioned2021-06-23T19:42:09Z
dc.date.available2021-06-23T19:42:09Z
dc.date.issued2015
dc.departmentBAİBÜ, Diş Hekimliği Fakültesi, Ağız Diş ve Çene Cerrahisi Ana Bilim Dalıen_US
dc.description.abstractBackground: Tooth avulsion is the most severe type of traumatic dental injuries and it results in the complete displacement of the tooth out of its socket in alveolar bone. Reimplantation of the tooth is considered to be a best treatment modality due to its biological and psychological advantages. Its prognosis depends on the extra alveolar time, the storage medium, and the patients general health. Objective: The aim of this study was to evaluate the effect of Capparis spinosa (C. spinosa) in maintaining the viability of human periodontal ligament (PDL) cells using a real-time cell analysis method. Materials and Methods: Periodontal ligament cells were obtained from healthy human third molars extracted for orthodontic purposes. The storage media tested were: Dulbeccos Modified Eagle Medium (DMEM), C. spinosa, Hanks Balanced Salt Solution (HBSS), and light milk. A real-time cell analyzer system was used to evaluate cell viability. After seeding cell suspensions into the wells of the E-plate 96, PDL cells were treated with each of tested media and monitored for every 5 min for 26 h. Statistical analysis of the data was accomplished using one-way analysis of variance complemented by the Tukey test. The level of significance was set at P < 0.05. Results: Dulbeccos Modified Eagle Medium (control) and C. spinosa groups had significantly higher cell index values compared with the HBSS and light milk (P < 0.05). Although, C. spinosa showed better results than DMEM (control), but this difference was not found statistically significant. Conclusion: Capparis spinosa can be a suitable, alternative storage medium for avulsed teeth.en_US
dc.identifier.doi10.4103/1119-3077.151766
dc.identifier.endpage399en_US
dc.identifier.issn1119-3077
dc.identifier.issue3en_US
dc.identifier.pmid25772925en_US
dc.identifier.scopus2-s2.0-84925808825en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage395en_US
dc.identifier.urihttps://doi.org/10.4103/1119-3077.151766
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8364
dc.identifier.volume18en_US
dc.identifier.wosWOS:000351753800017en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorOzan, Fatih
dc.institutionauthorOzan, Ülkü
dc.institutionauthorToptaş, Orçun
dc.language.isoenen_US
dc.publisherMedknow Publications & Media Pvt Ltden_US
dc.relation.ispartofNigerian Journal Of Clinical Practiceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAvulsionen_US
dc.subjectCapparis Spinosaen_US
dc.subjectCell Viabilityen_US
dc.subjectStorage Mediaen_US
dc.subjectReal Time Cell Analyzeren_US
dc.titleDynamic assessment of Capparis spinosa buds on survival of periodontal ligament cells using a real-time cell analysis methoden_US
dc.typeArticleen_US

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