Comparison of anti HCV signal-to-cutoff ratio with HCV RNA results
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Clinical Laboratory Publication
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: Anti-HCV antibody level screening is used in the diagnosis of HCV. However, a positive (S/Co > 1) result in the anti-HCV test does not always reflect true positivity. Antibody level results of S/Co ratio > 1 have to be validated through HCV RNA. In this study, we aimed to compare the signal-to-cutoff ratios of patients with positive (> 1) HCV antibody levels with the results of HCV RNA by PCR. Methods: In total, 17,021 samples were tested for anti HCV between January 2017 and December 2019. HCV antibody (anti HCV) was performed with a fully automated chemiluminescent microparticle immunoassay (CMIA, Abbot (R), Architect System). Real Time PCR test (Anatolia Geneworks HCV, Turkey) was used as nucleic acid amplification method. Results: Of the 17,021 patients, 16,706 (98.15%) tested negative and 315 (1.85%) tested positive in the anti-HCV assay. An additional HCV RNA test was requested for these 315 patients with positive anti-HCV assay results (S/Co >= 1) of which 23.81% (75/315) were positive for HCV RNA in serum, with a median (IQR): 5.43 log(10) IU/mL (4.75 - 6.01 log(10) IU/mL). Patients who tested positive for HCV RNA had significantly higher S/Co values compared to patients who tested negative (median (IQR): 13.38 (12.30 - 14.57) vs. 1.79 (1.34 - 1.79), p < 0.001). Conclusions: When S/Co ratios of patients who tested anti-HCV positive and HCV RNA positive were evaluated, it was assumed that high S/Co values were more relevant to true positivity. It was also concluded that low S/Co ratios needed to be verified through PCR.
Açıklama
Anahtar Kelimeler
Anti HCV, HCV RNA, Screening Test, Hepatitis-C, Virus
Kaynak
Clinical Laboratory
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
67
Sayı
2
Künye
Avcioglu, F., Behcet, M., & Kalaycioglu, O. (2021). Comparison of Anti HCV Signal-to-Cutoff Ratio with HCV RNA Results. Clinical laboratory, 67(2), 376-380.
