Isolation of the 3 beta-HSD promoter from Digitalis ferruginea subsp. ferruginea and its functional characterization in Arabidopsis thaliana

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dc.authorid0000-0001-6767-2539en_US
dc.authorid0000-0003-2933-5296en_US
dc.authorid0000-0003-2976-7224en_US
dc.authorid0000-0002-6786-3973en_US
dc.authorid0000-0001-6262-2866en_US
dc.contributor.authorAslam, Noreen
dc.contributor.authorSameeullah, Muhammad
dc.contributor.authorYıldırım, Muhammet
dc.contributor.authorBaloğlu, Mehmet Cengiz
dc.contributor.authorLossl, Andreas G.
dc.contributor.authorGürel, Ekrem
dc.contributor.authorYücesan, Bahtiyar Buhara
dc.date.accessioned2023-08-15T08:38:15Z
dc.date.available2023-08-15T08:38:15Z
dc.date.issued2022en_US
dc.departmentBAİBÜ, Lisansüstü Eğitim Enstitüsü, Fen Bilimleri, Biyoloji Ana Bilim Dalıen_US
dc.descriptionThe study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under the research grant number KBAG-115Z470 to EG.en_US
dc.description.abstractBackground Although members of the SDR gene family (short chain dehydrogenase) are distributed in kingdom of life, they have diverse roles in stress tolerance mechanism or secondary metabolite biosynthesis. Nevertheless, their precise roles in gene expression or regulation under stress are yet to be understood. Methods As a case study, we isolated, sequenced and functionally characterized the 3 beta-HSD promoter from Digitalis ferruginea subsp. ferruginea in Arabidopsis thaliana. Results The promoter fragment contained light and stress response elements such as Box-4, G-Box, TCT-motif, LAMP element, ABRE, ARE, WUN-motif, MYB, MYC, W box, STRE and Box S. The functional analysis of the 3 beta-HSD promoter in transgenic Arabidopsis seedlings showed that the promoter was expressed in cotyledon and root elongation zone in 2 days' seedlings. However, this expression was extended to hypocotyl and complete root in 6 days' seedlings. In 20 days-old seedlings, promoter expression was distributed to the whole seedling including hydathodes aperture, vascular bundle, shoot apical meristem, trichomes, midrib, leaf primordia, hypocotyl and xylem tissues. Further, expression of the promoter was enhanced or remained stable under the different abiotic stress conditions like osmotic, heat, cold, cadmium or low pH. In addition, the promoter also showed response to methyl jasmonate (MeJA) application. The expression could not be induced in wounded cotyledon most likely due to lack of interacting elements in the promoter fragment. Conclusions Taken together, the 3 beta-HSD promoter could be a candidate for the development of transgenic plants especially under changing environmental conditions.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [KBAG-115Z470]en_US
dc.identifier.citationAslam, N., Sameeullah, M., Yildirim, M., Baloglu, M. C., Yucesan, B., Lössl, A. G., ... & Gurel, E. (2022). Isolation of the 3β-HSD promoter from Digitalis ferruginea subsp. ferruginea and its functional characterization in Arabidopsis thaliana. Molecular Biology Reports, 49(7), 7173-7183.en_US
dc.identifier.doi10.1007/s11033-022-07634-4
dc.identifier.endpage7183en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue7en_US
dc.identifier.pmid35733064en_US
dc.identifier.scopus2-s2.0-85132730999en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage7173en_US
dc.identifier.urihttp://dx.doi.org/10.1007/s11033-022-07634-4
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11521
dc.identifier.volume49en_US
dc.identifier.wosWOS:000814480600014en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAslam, Noreen
dc.institutionauthorSameeullah, Muhammad
dc.institutionauthorYıldırım, Muhammet
dc.institutionauthorGürel, Ekrem
dc.institutionauthorYücesan, Bahtiyar Buhara
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitak[KBAG-115Z470]
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSDRen_US
dc.subject3 Beta-HSD Promoteren_US
dc.subjectFunctional Characterizationen_US
dc.subjectCallus-Culturesen_US
dc.subjectGene Promoteren_US
dc.subjectPlant-Growthen_US
dc.titleIsolation of the 3 beta-HSD promoter from Digitalis ferruginea subsp. ferruginea and its functional characterization in Arabidopsis thalianaen_US
dc.typeArticleen_US

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