Antiproliferative activity of some medicinal plants on human breast and hepatocellular carcinoma cell lines and their phenolic contents

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Küçük Resim

Tarih

2015

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Pharmacotherapy Group

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Purpose: To determine the phenolic composition and antiproliferative activity of 16 different extracts (hexane, dichloromethane, methanol and water) obtained from Bellis perennis, Convolvulus galaticus, Trifolium pannonicum and Lysimachia vulgaris on human breast cancer (MCF-7) and human hepatocellular carcinoma (HepG2/C3A) cell lines. Methods: The aerial parts of the plants were successively extracted with hexane, dichloromethane, methanol and water using a Soxhlet apparatus. The phenolic content of the plants were determined by plants by high performance liquid chromatography (HPLC) while their antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, a yellow tetrazole (MTT) assay. Results: Among the tested extracts, the methanol extract of B. perennis showed the best antiproliferative activity against MCF-7 cell line with IC50 (inhibiting 50 % of cell growth) value of 71.6 mu g/mL. Furthermore, the dichloromethane extract of C. galaticus showed the best anti-proliferative activity against HepG2/C3A cell line with IC50 of 57.3 mu g/mL. The HPLC data for the plant extracts showed the presence of the following phenolic compounds: gallic acid monohydrate, caffeic acid, rutin hydrate, luteolin-7-O-beta-D glucoside, kaempferol, myricetin, quercetin, coumarin and apigenin. Conclusion: The findings of this study indicate that there is some justification for the use of B. perennis and C. galaticus as traditional anticancer medicinal herbs.

Açıklama

Anahtar Kelimeler

Bellis Perennis, Convolvulus Galaticus, Trifolium Pannonicum Subsp Elongatum, Lysimachia Vulgaris, MCF-7, HepG2/C3A, Phenolics, Breast Cancer, Antiproliferative

Kaynak

Tropical Journal Of Pharmaceutical Research

WoS Q Değeri

Q4

Scopus Q Değeri

Q3

Cilt

14

Sayı

10

Künye