The role of oxidative stress in chemotherapy-induced gonadotoxicity in a rat model, and the protective effects of Nigella Sativa oil on oxidative stress, the anti-Mullerian hormone level, and apoptosis
Yükleniyor...
Dosyalar
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Verduci Publisher
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
OBJECTIVE: This study aimed to determine the role of oxidative stress (OS) in carboplatin-induced gonadotoxicity and whether Nigella Sativa oil (NSO), an herbal antioxidant, has a protective effect on ovarian apoptosis, OS, and the anti-Mullerian hormone (AMH) level in a rat model.
MATERIALS AND METHODS: The study included 24 adult female rats that were divided into 4 treatment groups. Group A saline + saline (sham group); group B: NSO + saline; group C: saline + carboplatin; group D: NSO + carboplatin. Saline, NSO, and carboplatin were administered intraperitoneally 24 and/or 48 h before sacrification as 4 mL/kg, 4 mL/kg, and 80 mg/kg, respectively. Apoptosis, OS parameters, and AMH were measured.
RESULTS: Oxidant levels and apoptosis were higher, whereas AMH and the antioxidants were lower in group C than in group A. Apoptosis, OS parameters, and AMH levels were negatively affected by chemotherapy (CTx) in group C whilst improvement in those parameters was observed in group D following NSO pretreatment. The levels of apoptosis and malondialdehyde (MDA), an OS parameter, in group D were lower than in group C as they declined from 34.3% to 8.65% (p = 0.002) and from 199.4 nmol/g tissue to 136.4 nmol/g tissue (p = 0.002), respectively. However, the slight increase in AMH level from 2.7 ng/mL to 3.5 ng/mL due to the NSO effect was not significant between groups C and D.
CONCLUSIONS: The present findings show that carboplatin has adverse effects on AMH, ovarian tissue apoptosis, and OS parameters. NSO pretreatment might protect ovarian tissue and decrease CTx-induced ovarian injury by decreasing OS and apoptosis, but the protective effect of NSO on AMH is limited.
Açıklama
This study was supported by Scientific Research Project, Giresun University, Turkey (Project grant number: SAG-BAP-A-140316-95).
Anahtar Kelimeler
Antioxidant, Apoptosis, Gonadotoxicity, Nigella Sativa Oil, Oxidative Stress, Ovarian Reserve
Kaynak
European Review for Medical and Pharmacological Sciences
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
27
Sayı
13
Künye
Cetinkaya, K., Atasever, M., Erisgin, Z., Sonmez, C., Ozer, C., Coskun, B., & Alisik, M. (2023). The role of oxidative stress in chemotherapyinduced gonadotoxicity in a rat model, and the protective effects of Nigella Sativa oil on oxidative stress, the anti-Müllerian hormone level, and apoptosis. European Review for Medical & Pharmacological Sciences, 27(13).
