The effects of leptin administration to amygdala region on anxiety, depression and learning behaviors: change in seretonine and glutamate levels
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-Blackwell
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
AIM: Leptin is a hormone secreted by adipose tissue and involved in modulation of food intake. In addition to these functions, it plays a role in various physiological events such as anxiety, depression and learning. The aim of this study was the investigation of effects of leptin administration to amygdala on anxiety, depression and learning behaviors. In addition, changing of serotonin and glutamate neurotransmitter levels interaction between behavior. METHODS: Presentstudy used Wistar albino rats. Animals were separated 3 groupssuch aslow dose (0.1µg/kg) and high dose (1µg/kg) leptin and saline (control) intraamigdalar administration group. In thisstudy, we planned two procedures. Firstly, a special cannula was implanted into amygdala region of rats and leptin and saline were injected in thisregion by infusion pump. After leptin injections, behavioral tests were performed. In the second study, the extracellular fluid was collected by microdialysis from the amygdale region.The levels of serotonin and glutamate were measured by HPLC. RESULTS: The first experiment demonstrated that in the open field, low dose leptin administrated rats spent more time at the center (F(2,57)= 3.97 p=0.02), showed more zone transition than control ones (F(2,57)= 3.22 p=0.04). In the elevated plus maze, high and low doses leptin administrated rats spent more time in the open arms(F(2,55)= 6.77 p=0.002) and also high dose leptin injected rats showed more frequency of entering open arms (F(2,55)= 9.97 p=0.001). In Porsolt test, low dose leptin administrated ratsshowed high mobility in center than sham group (F(2,55)= 4.30 p=0.02). In the Morris water maze, low dose leptin administrated rats spent more time over the platform (F(2,45)= 3.50 p=0.04). The second experiment demonstrated that serotonin and glutamate levels increased in the first hour after the leptin injections (p<0.05). CONCLUSIONS: Present study suggests that both high and low leptin administration to rat brain playsa role as anxiolytic and antidepression agents in rats
Açıklama
Anahtar Kelimeler
Anxiety, Depression and Learning Behaviors, Seretonine and Glutamate Levels, Amygdala Region, Leptin
Kaynak
Acta Physiologica
WoS Q Değeri
Q1
Scopus Q Değeri
Cilt
218
