Effect of montelukast on acute lung injury induced by intestinal ischemia and reperfusion in rats
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Dosyalar
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Carbone Editore
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aims: Lungs are the most affected remote organs after intestinal ischemia/reperfusion. The aim of our study is to investigate the effects of montelukast on intestinal ischemia/reperfusion-induced acute pulmonary injury, mast cells, and apoptosis. Materials and methods': Wistar Albino rats were used in the study. Control, intestinal ischemia/reperfusion (IIR), and IRR+montelukast groups, each comprising six rats, were constructed. Ischemia was induced by ligating the superior mesenteric artery for 60 min, followed by 60 min of reperfusion. Montelukast at a dose of 7 mg/kg was administered intraperitoneally 10 min before reperfusion. Lung tissues were fixed in neutral formalin. Paraffin sections were stained with hematoxylin-eosin and toluidine blue to assess mast cells. Pulmonary damage was graded, and mast cells were counted. Apoptotic cells were examined by terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) staining. Results: Thickening of the inter-alveolar wall, capillary congestion, haemorrhage, edema, and accumulation of numerous inflammatory cells around the vessels were observed in the lung specimens of the IIR group. Wall thickness, edema, haemorrhage, and inflammatory cell infiltration were less severe in the montelukast-treated group. The number of mast cells and apoptotic cells with positive TUNEL staining was found to be increased in the IRR group and decreased in the montelukast-treated group. Conclusion: Montelukast alleviated tissue damage and reduced the number of mast cells and apoptotic epithelial cells caused by IIR-induced acute pulmonary injury. These results suggest a new approach for the prevention or treatment of secondary lung injury.
Açıklama
Anahtar Kelimeler
Intestinal Ischemia/Reperfusion, Lung, Montelukast, Apoptosis, Mast Cells
Kaynak
Acta Medica Mediterranea
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
30
Sayı
2
