Inhibitory effects of regorafenib, a multiple tyrosine kinase inhibitor, on corneal neovascularization
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Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ijo Press
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
AIM: To evaluate the inhibitory effects of regorafenib (BAY 73 -4506), a multikinase inhibitor, on corneal neovascularization (NV). METHODS: Thirty adult male Sprague -Dawley rats weighing 250-300 g, were used. Corneal NV was induced by NaOH in the left eyes of each rat. Following the establishment of alkali burn, the animals were randomized into five groups according to topical treatment. Group 1 (n= 6) received 0.9% NaCl, Group 2 (n = 6) received dimethyl sulfoxide, Group 3 (n = 6) received regorafenib 1 mg/mL, Group 4 (n=6) received bevacizumab 5 mg/mL and Group 5 (n= 6) received 0.1% dexamethasone phosphate. On the 7d, the corneal surface covered with neovascular vessels was measured on photographs as the percentage of the cornea's total area using computer -imaging analysis. The corneas obtained from rats were semiquantitatively evaluated for caspase 3 and vascular endothelial growth factor by immunostaining. RESULTS: A statistically significant difference in the percent area of corneal NV was found among the groups (P <0.001). Although the Group 5 had the smallest percent area of corneal NV, there was no difference among Groups 3, 4 and 5 (P >0.005). There was a statistically significant difference among the groups in apoptotic cell density (P=0.002). The staining intensity of vascular endothelial growth factor in the epithelial and endothelial layers of cornea was significantly different among the groups (P<0.05). The staining intensity of epithelial and endothelial vascular endothelial growth factor was significantly weaker in Groups 3, 4 and 5 than in Groups 1 and 2. " CONCLUSION: Topical administration of regorafenib 1 mg/mL is partly effective for preventing alkali induced corneal NV in rats.
Açıklama
Anahtar Kelimeler
Corneal Neovascularization, Regorafenib, Tyrosine Kinase Inhibitor
Kaynak
International Journal Of Ophthalmology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
7
Sayı
2
