Monitoring of host suitability and defense-related genes in wheat to bipolaris sorokiniana

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dc.authorid0000-0002-7923-8892en_US
dc.authorid0000-0003-2562-4461en_US
dc.authorid0000-0002-7217-9092en_US
dc.authorid0000-0001-7934-2844en_US
dc.authorid0000-0002-1299-5733en_US
dc.contributor.authorAlkan, Mehtap
dc.contributor.authorBayraktar, Harun
dc.contributor.authorİmren, Mustafa
dc.contributor.authorÖzdemir, Fatih
dc.contributor.authorLahlali, Rachid
dc.contributor.authorÖzer, Göksel
dc.date.accessioned2023-09-28T11:22:35Z
dc.date.available2023-09-28T11:22:35Z
dc.date.issued2022en_US
dc.departmentBAİBÜ, Lisansüstü Eğitim Enstitüsü, Fen Bilimleri, Bitki Koruma Ana Bilim Dalıen_US
dc.descriptionThis study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK), grant TOVAG 119O956en_US
dc.description.abstractSpot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat worldwide. This study investigated the aggressiveness of B. sorokiniana isolates from different wheat-growing areas of Bolu province in Turkey on the cultivar Seri-82. Host susceptibility of 55 wheat cultivars was evaluated against the most aggressive isolate. Our results indicated that the cultivars Anafarta and Koc-2015 were the most resistant. A specific and sensitive qPCR assay was developed for detecting the pathogen in plant tissues and evaluating wheat plants with different resistance levels. Three primer sets, BsGAPDHF/BsGAPDHR, BsITSF/BsITSR, and BsSSUF/BsSSUR, were designed based on glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacers, and 18S rRNA loci of B. sorokiniana with detection limits of 1, 0.1, and 0.1 pg of pathogen DNA, respectively. The qPCR assay was highly sensitive and did not amplify DNA from the other closely related fungal species and host plants. The protocol differentiated wheat plants with varying degrees of resistance. The assay developed a useful tool for the quantification of the pathogen in the early stages of infection and may provide a significant contribution to a more efficient selection of wheat genotypes in breeding studies. In the present study, expression levels of PR proteins, phenylalanine ammonia-lyase, catalase, ascorbate peroxidase, and superoxide dismutase enzymes were upregulated in Anafarta (resistant) and Nenehatun (susceptible) cultivars at different post-infection time points, but more induced in the susceptible cultivar. The results showed considerable variation in the expression levels and timing of defense genes in both cultivars.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [TOVAG 119O956]en_US
dc.identifier.citationAlkan, M., Bayraktar, H., İmren, M., Özdemir, F., Lahlali, R., Mokrini, F., ... & Özer, G. (2022). Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana. Journal of Fungi, 8(2), 149.en_US
dc.identifier.doi10.3390/jof8020149
dc.identifier.endpage18en_US
dc.identifier.issn2309-608X
dc.identifier.issue2en_US
dc.identifier.pmid35205903en_US
dc.identifier.scopus2-s2.0-85124494870en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttp://dx.doi.org/10.3390/jof8020149
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11760
dc.identifier.volume8en_US
dc.identifier.wosWOS:000765090000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAlkan, Mehtap
dc.institutionauthorİmren, Mustafa
dc.institutionauthorÖzer, Göksel
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofJournal of Fungien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - İdari Personel ve Öğrencien_US
dc.relation.tubitak[TOVAG 119O956]
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDisease Reactionen_US
dc.subjectGene Expressionen_US
dc.subjectPathogen Quantificationen_US
dc.subjectPathogenesis-Related Proteinsen_US
dc.subjectSpot Blotch Diseaseen_US
dc.subjectExpression Profilesen_US
dc.titleMonitoring of host suitability and defense-related genes in wheat to bipolaris sorokinianaen_US
dc.typeArticleen_US

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