Monitoring of host suitability and defense-related genes in wheat to bipolaris sorokiniana
Yükleniyor...
Dosyalar
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat worldwide. This study investigated the aggressiveness of B. sorokiniana isolates from different wheat-growing areas of Bolu province in Turkey on the cultivar Seri-82. Host susceptibility of 55 wheat cultivars was evaluated against the most aggressive isolate. Our results indicated that the cultivars Anafarta and Koc-2015 were the most resistant. A specific and sensitive qPCR assay was developed for detecting the pathogen in plant tissues and evaluating wheat plants with different resistance levels. Three primer sets, BsGAPDHF/BsGAPDHR, BsITSF/BsITSR, and BsSSUF/BsSSUR, were designed based on glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacers, and 18S rRNA loci of B. sorokiniana with detection limits of 1, 0.1, and 0.1 pg of pathogen DNA, respectively. The qPCR assay was highly sensitive and did not amplify DNA from the other closely related fungal species and host plants. The protocol differentiated wheat plants with varying degrees of resistance. The assay developed a useful tool for the quantification of the pathogen in the early stages of infection and may provide a significant contribution to a more efficient selection of wheat genotypes in breeding studies. In the present study, expression levels of PR proteins, phenylalanine ammonia-lyase, catalase, ascorbate peroxidase, and superoxide dismutase enzymes were upregulated in Anafarta (resistant) and Nenehatun (susceptible) cultivars at different post-infection time points, but more induced in the susceptible cultivar. The results showed considerable variation in the expression levels and timing of defense genes in both cultivars.
Açıklama
This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK), grant TOVAG 119O956
Anahtar Kelimeler
Disease Reaction, Gene Expression, Pathogen Quantification, Pathogenesis-Related Proteins, Spot Blotch Disease, Expression Profiles
Kaynak
Journal of Fungi
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
8
Sayı
2
Künye
Alkan, M., Bayraktar, H., İmren, M., Özdemir, F., Lahlali, R., Mokrini, F., ... & Özer, G. (2022). Monitoring of host suitability and defense-related genes in wheat to Bipolaris sorokiniana. Journal of Fungi, 8(2), 149.
