Expression of NGF, MCP-1, uroplakin III, and NOS in bladder urothelium after partial urethral obstruction in rats

Basit Öğe Kaydı
dc.authorid0000-0001-5608-5742
dc.authorid0000-0002-8212-7149
dc.authorid0000-0002-7006-3125
dc.authorid0000-0001-6768-1275
dc.contributor.authorÖztürk, Hayrettin
dc.contributor.authorÇetinkaya, Ayhan
dc.contributor.authorDüzcü, Selma Erdoğan
dc.contributor.authorYis, Özgür Mehmet
dc.date.accessioned2021-06-23T19:53:48Z
dc.date.available2021-06-23T19:53:48Z
dc.date.issued2020
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractIntroduction Although several cytokines, chemokines, and growth factors have been suggested to play a role in the development of bladder fibrosis and functional changes, the mechanisms that are effective in the pathogenesis of partial bladder outlet obstruction (pB00)-induced bladder fibrosis are not well understood. Objective We investigated the expressions of nerve growth factor (NGF), monocyte chemoattractant protein-1 (MCP-1), uroplakin III (URPIII), inducible nitric oxide synthase (iNOS), and endothelial NOS (eNOS) that may be involved in fibrosis in rats with partial urethral obstruction for 1, 2 and 3 weeks, and the changes in the associated ischemic and inflammatory processes. After 1, 2, and 3 weeks of pB00, blood samples were collected for assessment of renal function from the rats under anesthesia. The bladders were dissected for the tissue antioxidant enzyme activities and lipid peroxidation, including malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant status (TAS) and total oxidant status (TOS). The immunohistochemical studies were performed. Histopathologically, the number of urothelial layers was calculated and the thickness of the detrusor smooth muscle and lamina propria were quantitatively measured. Additionally, the edema and congestion in the submucosa were evaluated. Study design Twenty-eight male Sprague-Dawley rats were used in this study. Three separate experimental groups of pB00 (1 week [n = 7], 2 weeks [n = 7], and 3 weeks [n = 7]) were created, with an additional sham-operated control group (n = 7). Results Study design Twenty-eight male Sprague-Dawley rats were used in this study. Three separate experimental groups of pB00 (1 week [n = 7], 2 weeks [n = 7], and 3 weeks [n = 7]) were created, with an additional sham-operated control group (n = 7). Results The MDA levels increased in pBOO groups. The SOD values were decreased in the pBOO group for 1 week, and higher in the 3-week pBOO group. The TAS levels were increased in the 3 week pB00 group. The TOS levels increased in the pBOO groups. The number of urothelial layers was decreased in pBOO groups. The lamina propria, the smooth muscle thickness, edema and congestion were increase in the 1 and 2 week pBOO groups. The NGF and MCP-1 expression was increased in the 1-week and 2-week pBOO groups. The expression of URPIII in the epithelium gradually increased in the pBOO groups. In the pBOO groups, iNOS expression in the epithelium cells was significantly elevated. However, the eNOS expression was also significantly increased in the 2 week pBOO group. Conclusion Our study shows that overexpression of immunohistochemical parameters together with the negative effects of ischemic and inflammatory processes that subjected to pB00 for 1, 2 and 3 weeks may play a potential role in detrusor fibrosis in the rat bladders induced by pB00. However, understanding of the immunohistochemical parameters investigated in this experimental study is limited, and further studies targeting their relationship to pB00 could help us develop new strategies.en_US
dc.identifier.doi10.1016/j.jpurol.2020.09.013
dc.identifier.issn1477-5131
dc.identifier.issn1873-4898
dc.identifier.issue6en_US
dc.identifier.pmid32994092en_US
dc.identifier.scopus2-s2.0-85091686632en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.jpurol.2020.09.013
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10266
dc.identifier.volume16en_US
dc.identifier.wosWOS:000600600300012en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÖztürk, Hayrettin
dc.institutionauthorDüzcü, Selma Erdoğan
dc.institutionauthorÇetinkaya, Ayhan
dc.institutionauthorYis, Özgür Mehmet
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofJournal Of Pediatric Urologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectUrethral Obstructionen_US
dc.subjectIschemic Damageen_US
dc.subjectNGFen_US
dc.subjectMCP-1en_US
dc.subjectUroplakin IIIen_US
dc.subjectNOSen_US
dc.titleExpression of NGF, MCP-1, uroplakin III, and NOS in bladder urothelium after partial urethral obstruction in ratsen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
hayrettin-ozturk.pdf
Boyut:
5.78 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Cerrahi Tıp Bilimleri Bölümü Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Temel Tıp Bilimleri Bölümü Koleksiyonu