Attachment, proliferation and collagen type I mRNA expression of human gingival fibroblasts on different biodegradable membranes

Basit Öğe Kaydı
dc.authorid0000-0002-7553-3915en_US
dc.contributor.authorHakkı, Sema Sezgin
dc.contributor.authorKorkusuz, Petek
dc.contributor.authorPuralı, Nuhan
dc.contributor.authorBozkurt, Buket
dc.contributor.authorKuş, Mahmut
dc.contributor.authorDuran, İsmet
dc.date.accessioned2021-06-23T19:35:11Z
dc.date.available2021-06-23T19:35:11Z
dc.date.issued2013
dc.description.abstractThe purpose of this study was to investigate adhesion, proliferation and type I collagen (COL I) mRNA expression of gingival fibroblasts on different membranes used in periodontal applications. Collagen (C), acellular dermal matrix (ADM) and polylactic acid; polyglycolic acid; lactide/glycolide copolymer (PLGA) biodegradable membranes were combined with gingival fibroblasts in culture and incubated for 48 h. Cell adhesion was examined with scanning electron and confocal microscopy. MTT assay was used to measure proliferation. COL I mRNA expression was assessed using quantitative-polymerase chain reaction (QPCR). The PLGA group exhibited the lowest cell survival on day 5 and 10, and lowest cell proliferation on days 5, 10 and 14. While cell proliferation was similar in C and ADM groups, the C membrane showed a slightly greater increase in viable cells to day 10. Confocal and scanning electron microscopy confirmed the results of proliferation and MTT assays. The highest COL I mRNA expression was noted in the PLGA membrane group when compared to the C (p<0.01) and ADM (p<0.05) membrane groups. These data revealed that adherence and proliferation of primary gingival fibroblasts on collagen-based C and ADM membranes is better than that seen with PLGA membranes, and thus may be preferable in the treatment of gingival recession defects.en_US
dc.identifier.doi10.3109/03008207.2013.801467
dc.identifier.endpage266en_US
dc.identifier.issn0300-8207
dc.identifier.issn1607-8438
dc.identifier.issue4-5en_US
dc.identifier.pmid23758269en_US
dc.identifier.scopus2-s2.0-84884226125en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage260en_US
dc.identifier.urihttps://doi.org/10.3109/03008207.2013.801467
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7731
dc.identifier.volume54en_US
dc.identifier.wosWOS:000324476600003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorDuran, İsmet
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofConnective Tissue Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAttachmenten_US
dc.subjectBiocompatibilityen_US
dc.subjectBiodegradable Membranesen_US
dc.subjectCell Proliferationen_US
dc.subjectCollagenen_US
dc.subjectGingival Fibroblastsen_US
dc.titleAttachment, proliferation and collagen type I mRNA expression of human gingival fibroblasts on different biodegradable membranesen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
sema-s.-hakki.pdf
Boyut:
1.13 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam metin/Full text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Periodontoloji Ana Bilim Dalı Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu