Stereological evidence of non-selective hippocampal neurodegeneration, IGF-1 depletion, and behavioral deficit following short term bilateral

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dc.authorid0000-0001-5026-4352en_US
dc.authorid0000-0001-9254-175Xen_US
dc.authorid0000-0002-0788-7164en_US
dc.authorid0000-0003-2141-3210en_US
dc.contributor.authorHamadi, Naserddine
dc.contributor.authorDeniz, Ömür Gülsüm
dc.contributor.authorIslam, Azim Ullah Shamsul
dc.contributor.authorAmir, Naheed
dc.contributor.authorMinhas, Saeed Tariq
dc.contributor.authorMadjid, Nather
dc.date.accessioned2023-09-29T13:31:00Z
dc.date.available2023-09-29T13:31:00Z
dc.date.issued2023en_US
dc.departmentBAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionThis study was funded by Faculty grant (NP14-42) from the College of Medicine and Health Sciences, UAE University.en_US
dc.description.abstractThe development of animal models to study cell death in the brain is a delicate task. One of the models, that was discovered in the late eighties, is the induction of neurodegeneration through glucocorticoid withdrawal by adrenalectomy in albino rats. Such a model is one of the few noninvasive models for studying neurodegeneration. In the present study, using stereological technique and ultrastructural examination, we aimed to investigate the impact of short-term adrenalectomy (2 weeks) on different hippocampal neuronal populations in Wistar rats. In addition, the underlying mechanism(s) of degeneration in these neurons were investigated by measuring the levels of insulin-like growth factor-1 (IGF-1) and beta-nerve growth factor (beta-NGF). Moreover, we examined whether the biochemical and histological changes in the hippocampus, after short-term adrenalectomy, have an impact on the cognitive behavior of Wistar rats. Stereological counting in the hippocampus revealed significant neuronal deaths in the dentate gyrus and CA4/CA3, but not in the CA2 and CA1 areas, 7 and 14 days post adrenalectomy. The ultrastructural examinations revealed degenerated and degenerating neurons in the dentate, as well as CA4, and CA3 areas, over the course of 3, 7 and 14 days. The levels of IGF-1 were significantly decreased in the hippocampus of ADX rats 24 h post adrenalectomy, and lasted over the course of two weeks. However, beta-NGF was not affected in rats. Using a passive avoidance task, we found a cognitive deficit in the ADX compared to the SHAM operated rats over time (3, 7, and 14 days). In conclusion, both granule and pyramidal cells were degenerated in the hippocampus following short-term adrenalectomy. The early depletion of IGF-1 might play a role in hippocampal neuronal degeneration. Consequently, the loss of the hippocampal neurons after adrenalectomy leads to cognitive deficits.en_US
dc.description.sponsorshipCollege of Medicine and Health Sciences, UAE University [NP14-42]en_US
dc.identifier.citationHamadi, N., Deniz, Ö. G., Issa, A. S. A., Islam, A. U. S., Amir, N., Minhas, S. T., ... & Adem, A. (2022). Stereological Evidence of Non-Selective Hippocampal Neurodegeneration, IGF-1 Depletion, and Behavioral Deficit following Short Term Bilateral Adrenalectomy in Wistar Rats. Biomolecules, 13(1), 22.en_US
dc.identifier.doi10.3390/biom13010022
dc.identifier.endpage19en_US
dc.identifier.issn2218-273X
dc.identifier.issue1en_US
dc.identifier.pmid36671407en_US
dc.identifier.scopus2-s2.0-85146556578en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttp://dx.doi.org/10.3390/biom13010022
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11779
dc.identifier.volume13en_US
dc.identifier.wosWOS:000917381400001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorDeniz, Ömür Gülsüm
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofBiomoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHippocampusen_US
dc.subjectNeurodegenerationen_US
dc.subjectAdrenalectomyen_US
dc.subjectNerve Growth-Factoren_US
dc.subjectGranule Cell Degeneratıonen_US
dc.subjectDentate Gyrusen_US
dc.titleStereological evidence of non-selective hippocampal neurodegeneration, IGF-1 depletion, and behavioral deficit following short term bilateralen_US
dc.typeArticleen_US

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