Characterization of biodegradable chitosan microspheres containing vancomycin and treatment of experimental osteomyelitis caused by methicillin-resistant staphylococcus aureus with prepared microspheres

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dc.authorid0000-0001-7069-0623en_US
dc.authorid0000-0002-0486-2252en_US
dc.authorid0000-0002-7426-0740
dc.authorid0000-0001-6973-473X
dc.authorid0000-0003-4897-6355
dc.contributor.authorCevher, Erdal
dc.contributor.authorOrhan, Zafer
dc.contributor.authorMulazimoğlu, Lütfiye
dc.contributor.authorŞensoy, Demet
dc.contributor.authorAlper, Murat
dc.contributor.authorYıldız, Ayça
dc.contributor.authorÖzsoy, Yıldız
dc.date.accessioned2021-06-23T19:19:00Z
dc.date.available2021-06-23T19:19:00Z
dc.date.issued2006
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractThe biodegradable chitosan microspheres containing vancomycin hydrochloride (VANCO) were prepared by spray drying method with different polymer:drug ratios ( 1: 1, 2:1, 3:1 and 4: 1). Thermal behaviour, particle size and distribution, morphological characteristics, drug content, encapsulation efficiency, in vitro release assessments of formulations have been carried out to obtain suitable formulation which shows sustained-release effect when implanted. Sterilized VANCO loaded microspheres were implanted to proximal tibia of rats with methicillin-resistant Staphylococcus aureus (MRSA) osteomyelitis. Intramuscular (IM) injection of VANCO for 21 days was applied to another group for comparison. After 3 weeks of treatment, bone samples were analysed with a microbiological assay. According to the results, encapsulation efficiency and yield of microspheres in all formulations were higher than 98% and 47%, respectively. Particle sizes of microspheres were smaller than 6 mu m. All microsphere formulations have shown sustained-release effect. In vitro drug release rate decreased due to the increase in polymer:drug ratio but no significant difference was seen between these results (p > 0.05). Based on our in vivo data, rats implanted VANCO-loaded chitosan inicrospheres and administered IM injection showed 3354 3366 and 52500 25635 colony forming, unit of MRSA in 1g bone samples (CFU/g), respectively. As a result, implanted VANCO-loaded microspheres were found to be more effective than IM route for the treatment of experimental osteomyelitis.en_US
dc.identifier.doi10.1016/j.ijpharm.2006.03.014
dc.identifier.endpage135en_US
dc.identifier.issn0378-5173
dc.identifier.issn1873-3476
dc.identifier.issue2en_US
dc.identifier.pmid16624509en_US
dc.identifier.scopus2-s2.0-33745222792en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage127en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijpharm.2006.03.014
dc.identifier.urihttps://hdl.handle.net/20.500.12491/5855
dc.identifier.volume317en_US
dc.identifier.wosWOS:000239293400004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorOrhan, Zafer
dc.institutionauthorAlper, Murat
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofInternational Journal Of Pharmaceuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVancomycinen_US
dc.subjectBiodegradable Microspheresen_US
dc.subjectChitosanen_US
dc.subjectSpray Drying Methoden_US
dc.subjectMethicillin-resistant Staphylococcus Aureusen_US
dc.subjectExperimental Osteomyelitisen_US
dc.titleCharacterization of biodegradable chitosan microspheres containing vancomycin and treatment of experimental osteomyelitis caused by methicillin-resistant staphylococcus aureus with prepared microspheresen_US
dc.typeArticleen_US

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