Molecular characterization of native entomopathogenic fungi from ambrosia beetles in hazelnut orchards of Turkey and evaluation of their in vitro efficacy
Yükleniyor...
Dosyalar
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Turkey is the world’s largest producer and exporter of hazelnut. Ambrosia
beetle species are the most common species of pests for hazelnut in the orchards of Turkey. These
beetles cause enormous economic losses by draining hazelnut branches and trees. The techniques
for managing ambrosia beetles are limited. The more effective and eco-friendly alternative control
methods, including the use of entomopathogenic fungi (EPF), should be included in integrated
pest management programs to suppress ambrosia beetle populations. The objectives of the current
study were (i) to isolate EPF from individual ambrosia beetles that were obtained from Turkey’s
main hazelnut production areas; (ii) to characterize EPF isolates using DNA sequencing and iPBS
profiling; and (iii) to assess the effectiveness of the isolates against three ambrosia beetle species under
laboratory conditions. A total of 47 EPF isolates were obtained from beetle cadavers and classified
into eight EPF species. For the first time, the primer binding site (PBS) marker system was used to
successfully discriminate among the EPF species. Some isolates caused 100% mortality of the beetle
species within 7 to 9 days, depending on the beetle species, demonstrating their effectiveness in
managing the pests. The major EPF species in this study provided an important basis for developing
bioproducts and a possible alternative approach in controlling these ambrosia beetles.
Abstract: Ambrosia beetles, Anisandrus dispar Fabricius, Xylosandrus germanus Blandford, and Xyleborinus
saxesenii Ratzeburg (Coleoptera: Curculionidae: Scolytinae) are among the most significant
hazelnut pests in Turkey. The control of these pests is difficult and expensive due to their biology.
The present study aimed to isolate entomopathogenic fungi (EPF) from A. dispar, X. germanus, and X.
saxesenii individuals that were obtained from the main hazelnut production areas of Turkey, characterize
the EPF isolates using internal transcribed spacer (ITS)-DNA sequencing and iPBS profiling, and
determine the efficacy of the isolates against A. dispar, X. germanus, and X. saxesenii under laboratory
conditions. Phylogenetic analyses based on ITS revealed that the 47 native isolates were Beauveria
bassiana (11), B. pseudobassiana (8), Cordyceps fumosorosea (6), Cordyceps farinosa (1), Akanthomyces
lecanii (13), Purpureocillium lilacinum (3), Clonostachys rosea (2) and Metarhizium anisopliae (3). For
the first time, the primer binding site (PBS) marker system, based on retrotransposons, was used
to discriminate successfully among the EPF species. Some isolates of B. bassiana, B. pseudobassiana,
C. fumosorosea, A. lecanii, and M. anisopliae caused 100% mortality of the beetle species within 7 to
9 days. The findings of this study indicated that some isolated entomopathogenic fungi provide an
essential basis for the development of bioproducts, as well as a promising alternative method for
controlling these ambrosia beetles.
Açıklama
Anahtar Kelimeler
Anisandrus Dispar, Xylosandrus Germanus, Xyleborinus Saxesenii, Biocontrol, Molecular Characterization, Ipbs Profiling, Insect-Pathogenic Fungi
Kaynak
Insects
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
13
Sayı
9
Künye
Kushiyev, R., Tunçer, C., Özdemir, İ. O., Erper, İ., Kalendar, R., Alkan, M., & Özer, G. (2022). Molecular characterization of native entomopathogenic fungi from ambrosia beetles in hazelnut orchards of turkey and evaluation of their in vitro efficacy. Insects, 13(9), 824.
