Selective amplification of plasmonic sensor signal for cortisol detection using gold nanoparticles

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Tarih

2022

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

MDPI

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Herein, gold nanoparticles (AuNP)-modified cortisol-imprinted (AuNP-MIP) plasmonic sensor was developed for signal amplification and real-time cortisol determination in both aqueous and complex solutions. Firstly, the sensor surfaces were modified with 3-(trimethoxylyl)propyl methacrylate and then pre-complex was prepared using the functional monomer N-methacryloyl-L-histidine methyl ester. The monomer solution was made ready for polymerization by adding 2-hydroxyethyl methacrylate to ethylene glycol dimethacrylate. In order to confirm the signal enhancing effect of AuNP, only cortisol-imprinted (MIP) plasmonic sensor was prepared without AuNP. To determine the selectivity efficiency of the imprinting process, the non-imprinted (AuNP-NIP) plasmonic sensor was also prepared without cortisol. The characterization studies of the sensors were performed with atomic force microscopy and contact angle measurements. The kinetic analysis of the AuNP-MIP plasmonic sensor exhibited a high correlation coefficient (R-2 = 0.97) for a wide range (0.01-100 ppb) with a low detection limit (0.0087 ppb) for cortisol detection. Moreover, the high imprinting efficiency (k ' = 9.67) of the AuNP-MIP plasmonic sensor was determined by comparison with the AuNP-NIP plasmonic sensor. All kinetic results were validated and confirmed by HPLC.

Açıklama

Anahtar Kelimeler

Cortisol Detection, Gold Nanoparticles, Molecular Imprinting, Plasmonic Sensor, Salivary Cortisol, Electrochemical Immunosensor

Kaynak

Biosensors-Basel

WoS Q Değeri

Q1

Scopus Q Değeri

Q1

Cilt

12

Sayı

7

Künye

Yılmaz, G. E., Saylan, Y., Göktürk, I., Yılmaz, F., & Denizli, A. (2022). Selective amplification of plasmonic sensor signal for cortisol detection using gold nanoparticles. Biosensors, 12(7), 482.