Effects of artificial ultraviolet C radiation on several blood and urine parameters related to renal and hepatic functions in albino mice
Küçük Resim Yok
Tarih
2007
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Natl Veterinary Research Inst
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The effects of artificial UVC radiation on some blood and urine parameters related to hepatic and renal functions in mice radiated with 30-watt UVC lamp (254 nm and 0.00014 J/cm(2)/s) were examined. Sixty female mice (Swiss Albino) were used as test animals. The animals were fed a mouse diet and water ad libitum and then exposed to artificial UVC for 8 h daily. Blood and urine samples were taken before radiation (control) and at the 7(th), 15(th), 30(th), 45(th), 60(th), and 75(th) d after radiation exposure. The samples were collected in different pools. Urea, creatinine, cholesterol, ALT, AST, ALP, and GGT levels were determined in serum, and protein electrophoresis was made (SDS-PAGE) in urine samples. Significant increase (P<0.001) in serum urea values were observed on the 15(th) and 30(th) d of the exposure. Urea levels returned to the initial values. Increase in creatinine levels after 7(th) d of exposure and decrease until the 60th d (significant on the 15(th), and 45(th) d) were found. Creatinine levels increased again on the 75(th) d (P<0.001). Serum cholesterol and AST values were significantly increased (P<0.01) during the whole period of the exposure, except the 45(th) d. Serum ALT levels were not significantly affected during 30(th) d exposure. They decreased on the 45(th) d (P<0.01) and then increased on the 75(th) d (P<0.01). Serum GGT and ALP levels were decreased during the whole period of the exposure (P<0.01), and GGT activity was at immeasurable levels on the 30(th), 45(th), and 60(th) d. Proteinuria, determined by microscopic observation Of Urine sediment, and the increase in serum urea and creatinine, observed on the 7(th), and 75(th) d of the exposure, were considered a renal disorder, and according to the electroplioretical pattern Of Urine proteins (SDS-PAGE) were defined as mostly glomerulo-tubular type and glomerular type. This indicates that artificial UVC radiation significantly affected the renal function (P<0.01), while the hepatic functions were affected significantly (P<0.01) just on the 75(th) d of the exposure., Concerning the data, which coincide one on the top of the other, there was the opinion that the kidneys and lk,cr showed different sensitivity to artificial UVC radiation.
Açıklama
Anahtar Kelimeler
Mice, UVC-radiation, Hepatic function, Renal function, Urine proteins
Kaynak
Bulletin Of The Veterinary Institute In Pulawy
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
51
Sayı
2
