Hydrogen peroxide-induced antioxidant activities and cardiotonic glycoside accumulation in callus cultures of endemic digitalis species

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dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0002-9324-747Xen_US
dc.contributor.authorCingöz, Günce Şahin
dc.contributor.authorVerma, Sandeep Kumar
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:35:36Z
dc.date.available2021-06-23T19:35:36Z
dc.date.issued2014
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractThe effect of hydrogen peroxide (H2O2) on callus cultures of four Digitalis species (Digitalis lamarckii, Digitalis trojana, Digitalis davisiana and Digitalis cariensis) increased catalase (CAT), superoxide dismutase (SOD), total phenolic, proline activity and cardiotonic glycoside production. Callus derived from hypocotyl explants was cultured on Murashige and Skoog medium supplemented with 0.25 mg L-1 indole-3-acetic acid (IAA) and 0.5 mg L-1 thidiazuron (TDZ). After a month of culture, callus was transferred to MS medium containing 10 mM H2O2 and then incubated for 6 h. The amount of five cardenolides (Lanatoside C, Digitoxin, Digoxigenin, Gitoxigenin and Digoxin) as well as CAT, SOD, total phenolic, proline activity from Digitalis species were compared. No digoxin was detected in all treatments and control groups. The total cardenolides estimated were in the order of D. lamarckii (586.65 mu g g(-1) dw), D. davisiana (506.79 mu g g(-1) dw), D. cariensis (376.60 mu g g(-1) dw) and D. trojana (282.39 mu g g(-1) dw). It was clear that H2O2 pre-treatment resulted in an increase in enzymatic and nonenzymatic antioxidants. However, a significant negative relationship between cardenolides production and overall activities of CAT, SOD, total phenolic and proline was evident. The described protocol here will be useful for the development of new strategies for a large-scale production of cardenolides. (C) 2014 Elsevier Masson SAS. All rights reserved.en_US
dc.identifier.doi10.1016/j.plaphy.2014.05.008
dc.identifier.endpage94en_US
dc.identifier.issn0981-9428
dc.identifier.pmid24915111en_US
dc.identifier.scopus2-s2.0-84901981643en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage89en_US
dc.identifier.urihttps://doi.org/10.1016/j.plaphy.2014.05.008
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7825
dc.identifier.volume82en_US
dc.identifier.wosWOS:000340698800011en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorCingöz, Günce Şahin
dc.institutionauthorVerma, Sandeep Kumar
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevieren_US
dc.relation.ispartofPlant Physiology And Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntioxidant Activityen_US
dc.subjectCardenolides Accumulationen_US
dc.subjectDigitalis L.en_US
dc.subjectFoxgloveen_US
dc.subjectHydrogen Peroxideen_US
dc.titleHydrogen peroxide-induced antioxidant activities and cardiotonic glycoside accumulation in callus cultures of endemic digitalis speciesen_US
dc.typeArticleen_US

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