Identification of 30-kDa heat shock protein gene in Trichophyton rubrum

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dc.contributor.authorBehzadi, Elham
dc.contributor.authorBehzadi, Payam
dc.contributor.authorSırmatel, Fatma
dc.date.accessioned2021-06-23T19:26:17Z
dc.date.available2021-06-23T19:26:17Z
dc.date.issued2009
dc.departmentBAİBÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractSmall heat shock proteins (sHSPs) are chaperones that are crucial in the heat shock response but also have important non-stress roles within the cell. HSP70 in Trichophyton rubrum is already detected and carefully characterised; however, no study was carried out for HSP30 in this pathogenic fungus. In the present study, T. rubrum was obtained from patients with dermatophytosis and cultured in appropriate conditions. High-molecular-weight DNA was extracted using standard extraction methods. Pairs of 21 nt primers were designed from highly conserved regions of the similar genes in other eukaryotic cells. Mentioned primers were utilised in PCR using isolated genomic DNA and extracted RNA templates of T. rubrum. The PCR fragments were then sequenced and 415 nucleotides of HSP30 in this pathogenic fungus were detected; the open reading frame had 156 nucleotides and was coding 51 amino acids. This gene (called TrHSP30) is registered in GenBank at National Center for Biotechnology Information (NIH, USA) database. Detection of TrHSP30 gene may open the way to determination of its possible role in the pathogenesis of dermatophyte infections due to T. rubrum.en_US
dc.identifier.doi10.1111/j.1439-0507.2008.01561.x
dc.identifier.endpage238en_US
dc.identifier.issn0933-7407
dc.identifier.issn1439-0507
dc.identifier.issue3en_US
dc.identifier.pmid18643918en_US
dc.identifier.scopus2-s2.0-63849237977en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage234en_US
dc.identifier.urihttps://doi.org/10.1111/j.1439-0507.2008.01561.x
dc.identifier.urihttps://hdl.handle.net/20.500.12491/6487
dc.identifier.volume52en_US
dc.identifier.wosWOS:000264820200005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBehzadi, Elham
dc.institutionauthorSırmatel, Fatma
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofMycosesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTrhsp30en_US
dc.subjectTrichophyton Rubrumen_US
dc.subjectPCRen_US
dc.subjectDermatophytosisen_US
dc.subjectHeat Shock Proteinsen_US
dc.titleIdentification of 30-kDa heat shock protein gene in Trichophyton rubrumen_US
dc.typeArticleen_US

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