Analytical evaluation of vitamin d measurements in routine laboratories

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dc.authorid0000-0002-7006-3125en_US
dc.authorid0000-0002-4060-3354en_US
dc.contributor.authorYis, Özgür Mehmet
dc.contributor.authorBuğdaycı, Güler
dc.contributor.authorSönmez, Çiğdem A.
dc.contributor.authorYıldız, Rabia N.
dc.date.accessioned2021-06-23T19:52:46Z
dc.date.available2021-06-23T19:52:46Z
dc.date.issued2019
dc.departmentBAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground: Its increasing clinical importance has turned 25-hydroxy-Vitamin D (25(OH)D) into an indispensable test in clinical laboratories. In this study, we aimed to analyze the analytical performances of two widely used immunoassays, namely new restandardized Abbott product Architect 25-OH Vitamin D test and the Beckman Coulter product Access 25(OH) Vitamin D Total Test by making comparisons with the reference method liquid chromatography tandem mass spectrometry (LC-MS/MS). Methods: The new restandardized Architect I2000SR System (Abbott Laboratories, IL, USA; ref 5P02) and Access2 (Beckman Coulter, Brea, CA, USA; ref B24838) immunoassay were compared with LC-MS/MS (Shimadzu LCMS-8030, Kyoto, Japan) method 25(OH)D test for precision and reproducibility in 90 serum samples. In comparison with reference method, Deming Regression analysis and Bland Altman graphs were used. Results: Within run coefficient of variation (CV%) for Architect was found to be lower than 3.1%. Within run coefficient of variation (CV%) for Access2 was lower than 7.04%. When compared with LC-MS/MS, R value of Architect 25-OH Vitamin D kit was 0.911 (intercept 1.62, slope 1.06), mean bias was -0.04% and for Access 25(OH) Vitamin D Total kit, R value was 0.841 (intercept 9.43, slope 0.92) and mean bias of 6.9%. Conclusions: When renewed 5P02 Abbott Architect 25(OH)D and Beckman Coulter Access2 25(OH)D Total tests were compared with LC-MS/MS method in our study, they were found to have appropriate analytical values.en_US
dc.identifier.doi10.7754/Clin.Lab.2018.180731
dc.identifier.endpage272en_US
dc.identifier.issn1433-6510
dc.identifier.issue3en_US
dc.identifier.pmid30868850en_US
dc.identifier.scopus2-s2.0-85062884909en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage267en_US
dc.identifier.urihttps://doi.org/10.7754/Clin.Lab.2018.180731
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10203
dc.identifier.volume65en_US
dc.identifier.wosWOS:000460916300008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorYis, Özgür Mehmet
dc.institutionauthorBuğdaycı, Güler
dc.language.isoenen_US
dc.publisherClin Lab Publen_US
dc.relation.ispartofClinical Laboratoryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVitamin Den_US
dc.subjectLuminescent Measurementsen_US
dc.subjectChemistry Methodsen_US
dc.subjectAnalytical Testen_US
dc.subjectRoutine Diagnosticen_US
dc.titleAnalytical evaluation of vitamin d measurements in routine laboratoriesen_US
dc.typeArticleen_US

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