Effect of partial outlet obstruction on nitrotyrosine content and distribution within the rabbit bladder

Basit Öğe Kaydı
dc.authorid0000-0002-2577-8711
dc.contributor.authorLevin, Robert M.
dc.contributor.authorAğartan, Canan Aldırmaz
dc.contributor.authorLeggett, Robert E.
dc.contributor.authorWhitbeck, Catherine
dc.contributor.authorChichester, Paul
dc.contributor.authorNeuman, Paul
dc.contributor.authorJohnson, Arnold
dc.date.accessioned2021-06-23T19:18:18Z
dc.date.available2021-06-23T19:18:18Z
dc.date.issued2005
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractPurpose: Evidence indicates that free radicals are etiological factors in obstructive bladder disease. However, it is not clear which species of reactive oxygen or nitrogen species mediate the damage. The current studies were designed to determine if partial outlet obstruction in rabbits results in the generation of nitrotyrosine (NT). Materials and methods: Sixteen rabbits were separated into four groups of four. The rabbits in groups 1 and 2 underwent sham operation while rabbits in groups 3 and 4 underwent partial outlet obstruction. The rabbits in groups 1 and 3 were evaluated after 1 week of obstruction and the rabbits in groups 2 and 4 were evaluated after 2 weeks of obstruction. A separate group of four controls were evaluated simultaneously with the sham and obstructed rabbits. Four rabbits from each group were evaluated after 1 and 2 weeks of obstruction. Four control rabbits were also evaluated. Isolated strips were evaluated for contractile responses and NT content of the mucosa and muscle were quantitated by Western blot analysis. Results: (1) The mucosa contains both 42 and 62 kD proteins exhibiting a strong nitrotyrosine signal; the muscle presents a signal only at 62 kD. (2) The sham operations had no effect on nitrotyrosine distribution or content. (3) The nitrotyrosine of both mucosal proteins and the muscle protein are increased in the 1 week obstructed bladder; whereas, only the 62 kD signal is increased in the two week obstructed bladder mucosa. (4) The contractile response to FS are reduced to a significantly greater degree than the responses to carbachol, KCl, or ATP. Conclusions: These studies clearly demonstrated that partial outlet obstruction in rabbits results in significant increases in nitrotyrosine within the bladder and may contribute to the contractile dysfunctions mediated by partial outlet obstruction.en_US
dc.identifier.doi10.1007/s11010-005-4061-5
dc.identifier.endpage148en_US
dc.identifier.issn0300-8177
dc.identifier.issue1-2en_US
dc.identifier.pmid16132695en_US
dc.identifier.scopus2-s2.0-24144487522en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage143en_US
dc.identifier.urihttps://doi.org/10.1007/s11010-005-4061-5
dc.identifier.urihttps://hdl.handle.net/20.500.12491/5693
dc.identifier.volume276en_US
dc.identifier.wosWOS:000231220500018en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAğartan, Canan Aldırmaz
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular And Cellular Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBladderen_US
dc.subjectRabbiten_US
dc.subjectNitric Oxide Synthaseen_US
dc.subjectNitrotyrosineen_US
dc.titleEffect of partial outlet obstruction on nitrotyrosine content and distribution within the rabbit bladderen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
robert-levin.pdf
Boyut:
1.24 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam metin/ Full text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Cerrahi Tıp Bilimleri Bölümü Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu