High quality human sperm selection for IVF: A study on sperm chromatin condensation

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dc.authorid0000-0002-1065-3254
dc.authorid0000-0001-5400-0012
dc.contributor.authorŞaylan, Aslıhan
dc.contributor.authorErimşah, Sevilay
dc.date.accessioned2021-06-23T19:50:45Z
dc.date.available2021-06-23T19:50:45Z
dc.date.issued2019
dc.departmentBAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractThe study consisted of semen samples of 20 male individuals who applied to Abant Izzet Baysal University Faculty of Medicine and participated in a spermiogram. The aim of this study was to determine how to obtain the healthiest spermatozoa by employing a variety of swim-up methods over differing time periods and without the use of centrifuge. Ejaculate samples were taken from the 20 patients and each patient's homogenized semen sample was divided into 4 groups without centrifugation. Group 1 was taken as the sample of untreated semen. For the other 3 groups, 250 mu l of medium was added in the semen samples. Afterwards, the samples were kept at 37 degrees C for different time periods, 30 min for Group 2, 60 min for Group 3 and 90 min for Group 4 in order for the spermatozoa to swim to the media in the upper layer. At the end of the periods, 10 mu l of propagation preparations were prepared from the swim-up fluid. Using Aniline Blue for chromatin condensation analysis, two hundred cells were immunostained by Caspase 3 for apoptotic analysis. Subsequently, the result of the four groups were compared for each test. The spermatozoa obtained at the end of the 30 min. of swim-up was compared to the spermatozoa obtained from the swim-up of 60 min., the swim-up of 90 min. It was found that the control group had statistically significant lower rates of apoptosis and was healthier in terms of chromatin integrity. The swim-up method without centrifugation is the best suited sperm preparation, based on sperm DNA integrity and sperm chromatin condensation.en_US
dc.identifier.doi10.1016/j.acthis.2019.07.006
dc.identifier.endpage803en_US
dc.identifier.issn0065-1281
dc.identifier.issn1618-0372
dc.identifier.issue7en_US
dc.identifier.pmid31345569en_US
dc.identifier.scopus2-s2.0-85069664562en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage798en_US
dc.identifier.urihttps://doi.org/10.1016/j.acthis.2019.07.006
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9855
dc.identifier.volume121en_US
dc.identifier.wosWOS:000499933800005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorŞaylan, Aslıhan
dc.institutionauthorErimşah, Sevilay
dc.language.isoenen_US
dc.publisherElsevier Gmbhen_US
dc.relation.ispartofActa Histochemicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectInfertilityen_US
dc.subjectSpermatozoa Selectionen_US
dc.subjectChromatin Integrityen_US
dc.subjectDNA Fragmentationen_US
dc.titleHigh quality human sperm selection for IVF: A study on sperm chromatin condensationen_US
dc.typeArticleen_US

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