Mapping of microsatellite instability in endoscopic normal colon

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dc.authorid0000-0002-0901-9192en_US
dc.authorid0000-0002-2408-5294en_US
dc.contributor.authorTuğ, Esra
dc.contributor.authorBalaban, Hatice Yasemin
dc.contributor.authorŞahin, Ebru Kaplan
dc.date.accessioned2021-06-23T19:33:50Z
dc.date.available2021-06-23T19:33:50Z
dc.date.issued2012
dc.departmentBAİBÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractGenomic instability in colorectal cancer (CRC) occurs as either microsatellite instability (MSI) or chromosomal instability. The present study was aimed at examining the MSI for the MLH1 and MSH2 genes in normal colon and polyps, if detected. Four segments of the colon were sampled in 102 subjects during colonoscopy. DNA samples were analyzed for the MSI status according to the Bethesda consensus panel. Family history of any type of cancer or for colon cancer was present in 44.8% and 9.4% of the individuals, respectively. Forty-eight percent of individuals were microsatellite stable for all five markers at all locations, 20% had low MSI status (MSI-L), and 32% had high MSI status (MSI-H). The frequencies of MSI markers differed significantly from each other (p = 0.003). The most frequent positive marker was D17S250. This is the first study which revealed that MSI is present in endoscopically normal-looking colon of normal individuals and, more frequently, in individuals with family histories of CRC. The detection of very early-stage CRC is possible by MSI analysis of DNA mismatch repair genes in colon tissues. This study has revealed crucial information for the use of molecular tests in CRC screening, such as high frequencies of MSI in endoscopically normal colon, which might cause false positivity.en_US
dc.identifier.doi10.1089/gtmb.2011.0219
dc.identifier.endpage395en_US
dc.identifier.issn1945-0265
dc.identifier.issn1945-0257
dc.identifier.issue5en_US
dc.identifier.pmid22224632en_US
dc.identifier.scopus2-s2.0-84861499377en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage388en_US
dc.identifier.urihttps://doi.org/10.1089/gtmb.2011.0219
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7218
dc.identifier.volume16en_US
dc.identifier.wosWOS:000304448300015en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBalaban, Hatice Yasemin
dc.institutionauthorŞahin, Ebru Kaplan
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofGenetic Testing And Molecular Biomarkersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEndoscopic Normal Colonen_US
dc.titleMapping of microsatellite instability in endoscopic normal colonen_US
dc.typeArticleen_US

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