Inducible expression of human papillomavirus-16 L1 capsomeres in the plastomes of Nicotiana tabacum: Transplastomic plants develop normal flowers and pollen

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dc.authorid0000-0001-6767-2539en_US
dc.contributor.authorLatif, Sara
dc.contributor.authorGottschamel, Johanna
dc.contributor.authorSyed, Tahira
dc.contributor.authorSameeullah, Muhammad
dc.contributor.authorYounus, Iqra
dc.contributor.authorGull, Kehkshan
dc.date.accessioned2023-07-20T08:58:12Z
dc.date.available2023-07-20T08:58:12Z
dc.date.issued2022en_US
dc.departmentBAİBÜ, Rektörlük, Yenilikçi Gıda Teknolojileri Geliştirme Uygulama ve Araştırma Merkezien_US
dc.description.abstractHuman papillomavirus type-16 (HPV-16) is the major HPV type involved in causing cervical cancer among women. The disease burden is high in developing and underdeveloped countries. Previously, the constitutive expression of HPV-16 L1 protein led to male sterility in transplastomic tobacco plants. Here, the HPV-16 L1 gene was expressed in chloroplasts of Nicotiana tabacum under the control of an ethanol-inducible promoter, trans-activated by nucleus-derived signal peptide. Plants containing nuclear component were transformed with transformation vector pEXP-T7-L1 by biolistic gun. The transformation and homoplasmic status of transformed plants was verified by polymerase chain reaction and Southern blotting, respectively. Protein was induced by spraying 5% ethanol for 7 consecutive days. The correct folding of L1 protein was confirmed by antigen-capture ELISA using a conformation-specific antibody. The L1 protein accumulated up to 3 mu g/g of fresh plant material. The L1 protein was further purified using affinity chromatography. All transplastomic plants developed normal flowers and produced viable seeds upon self-pollination. Pollens also showed completely normal structure under light microscope and scanning electron microscopy. These data confirm the use of the inducible expression as plant-safe approach for expressing transgenes in plants, especially those genes that cause detrimental effects on plant growth and morphology.en_US
dc.identifier.citationLatif, S., Gottschamel, J., Syed, T., Younus, I., Gull, K., Sameeullah, M., ... & Waheed, M. T. (2022). Inducible expression of human papillomavirus‐16 L1 capsomeres in the plastomes of Nicotiana tabacum: Transplastomic plants develop normal flowers and pollen. Biotechnology and applied biochemistry, 69(2), 596-611.en_US
dc.identifier.doi10.1002/bab.2136
dc.identifier.endpage611en_US
dc.identifier.issn0885-4513
dc.identifier.issn1470-8744
dc.identifier.issue2en_US
dc.identifier.pmid33650709en_US
dc.identifier.scopus2-s2.0-85102751449en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage596en_US
dc.identifier.urihttp://dx.doi.org/10.1002/bab.2136
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11318
dc.identifier.volume69en_US
dc.identifier.wosWOS:000630599400001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSameeullah, Muhammad
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofBiotechnology and Applied Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiopharmaceuticalsen_US
dc.subjectCapsomeresen_US
dc.subjectCervical Canceren_US
dc.subjectPlastid Transgenesen_US
dc.subjectCancer Incidenceen_US
dc.subjectTobaccoen_US
dc.titleInducible expression of human papillomavirus-16 L1 capsomeres in the plastomes of Nicotiana tabacum: Transplastomic plants develop normal flowers and pollenen_US
dc.typeArticleen_US

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