Identification of conserved miRNA molecules in einkorn Wheat (Triticum Monococcum subsp. Monococcum) by using small RNA sequencing analysis

dc.authorid0000-0003-0097-1125
dc.authorid0000-0003-2944-5807
dc.authorid0000-0001-6747-2852
dc.authorid0000-0002-0337-0520
dc.authorid0000-0002-5677-2347
dc.contributor.authorÜnlü, Ercan Selçuk
dc.contributor.authorBataw, Sara
dc.contributor.authorŞen, Didem Aslan
dc.contributor.authorŞahin, Yunus
dc.contributor.authorZencirci, Nusret
dc.date.accessioned2021-06-23T18:40:11Z
dc.date.available2021-06-23T18:40:11Z
dc.date.issued2018
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Kimya Bölümüen_US
dc.departmentBAİBÜ, Lisansüstü Eğitim Enstitüsü, Fen Bilimleri, Biyoloji Ana Bilim Dalı
dc.description.abstractTriticum monococcum subsp. monococcum as a first cultivated diploid wheat species possesses desirable agronomic and quality characteristics. Drought and salinity are the most dramatic environmental stress factors that have serious impact on yield and quality of crops; however, plants can use alternative defense mechanisms against these stresses. The posttranscriptional alteration of gene expression by microRNAs (miRNAs) is one of the most conserved mechanisms. In plant species including wheat genomes, miRNAs have been implicated in the management of salt and drought stress; however, studies on einkorn wheat (Triticum monococcum subsp. monococcum) are not yet available. In this study, we aimed to identify conserved miRNAs in einkorn wheat using next generation sequencing technology and bioinformatics analysis. In order to include a larger set of miRNAs, small RNA molecules from pooled plant samples grown under normal, drought, and salinity conditions were used for the library preparation and sequence analysis. After bioinformatics analysis, we identified 167 putative mature miRNA sequences belonging to 140 distinct miRNA families. We also presented a comparative analysis to propose that miRNAs and their target genes were involved in salt and drought stress control in addition to a comprehensive analysis of the scanned target genes in the T. aestivum genome.en_US
dc.identifier.doi10.3906/biy-1802-3
dc.identifier.endpage536en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85060726522en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage527en_US
dc.identifier.trdizinid349231en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TXpRNU1qTXhNUT09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3002
dc.identifier.volume42en_US
dc.identifier.wosWOS:000452776900008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorÜnlü, Ercan Selçuk
dc.institutionauthorBataw, Sara
dc.institutionauthorŞen, Didem Aslan
dc.institutionauthorŞahin, Yunus
dc.institutionauthorZencirci, Nusret
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMicrornaen_US
dc.subjectWheat
dc.subjectPerl, Mfold
dc.subjectSmall Rnas
dc.titleIdentification of conserved miRNA molecules in einkorn Wheat (Triticum Monococcum subsp. Monococcum) by using small RNA sequencing analysisen_US
dc.typeArticleen_US

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