Evaluation of anti-inflammatory and antiapoptotic effects of bone marrow and adipose-derived mesenchymal stem cells in acute alkaline corneal burn

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dc.authorid0000-0003-2940-3172
dc.contributor.authorDinç, Erdem
dc.contributor.authorDursun, Özer
dc.contributor.authorYılmaz, Gülsen
dc.contributor.authorKurt, Akif Hakan
dc.contributor.authorAyaz, Lokman
dc.contributor.authorVatansever, Mustafa
dc.contributor.authorÖzer, Ömer
dc.date.accessioned2021-06-23T19:55:12Z
dc.date.available2021-06-23T19:55:12Z
dc.date.issued2021
dc.departmentBAİBÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractPurpose: The aim of the present study is to comparatively evaluate the anti-inflammatory and antiapoptotic effects of bone marrow and adipose-derived mesenchymal stem cells (MSCs) applied subconjunctivally after alkaline corneal burn. Methods: Thirty-two rats were divided into 4 groups and included in the study (n = 8). While no intervention was made in the control group, a chemical burn was created by applying 4 mu L of NaOH soaked in 6 mm filter paper to the right eye of each subject in the other groups under general anesthesia. While only subconjunctival 0.1 mL phosphate-buffered saline (PBS) was injected to in the group 1, 2 x 10(6) adipose or bone marrow-derived MSC in 0.1 mL PBS was applied subconjunctivally to the subjects in the remaining groups (Group 2 and 3, respectively). Tissue samples were collected for histological analysis on the third day after the burn. Tissue samples were evaluated light microscopically and immunohistochemically stained for interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha (TNF-alpha), caspase-3 (Cas-3), and CD68. Results: The IL-1 beta and TNF-alpha staining scores and the number of CD68- and Cas-3-positive stained cells were significantly lower in the groups given bone marrow and adipose-derived MSC compared to the alkaline burn group (P < 0.0001, for all parameters). Epithelial IL-1 beta and TNF-alpha staining scores were significantly lower in the bone marrow-derived MSC group compared to the adipose-derived MSC group (P < 0.0001, for all parameters). Conclusions: The presented study shows that both bone-marrow and adipose-derived MSCs support wound healing in the corneal tissue and strongly suppress the inflammation occured in the tissue.en_US
dc.identifier.doi10.1089/jop.2020.0103
dc.identifier.endpage34en_US
dc.identifier.issn1080-7683
dc.identifier.issn1557-7732
dc.identifier.issue1en_US
dc.identifier.pmid33275515en_US
dc.identifier.scopus2-s2.0-85099732659en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage24en_US
dc.identifier.urihttps://doi.org/10.1089/jop.2020.0103
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10751
dc.identifier.volume37en_US
dc.identifier.wosWOS:000596400300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKurt, Akif Hakan
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofJournal Of Ocular Pharmacology And Therapeuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlkaline Burnen_US
dc.subjectApoptosisen_US
dc.subjectChemical Corneal Burnen_US
dc.subjectInflammationen_US
dc.subjectMesenchymal Stem Cellen_US
dc.subjectWound Healingen_US
dc.titleEvaluation of anti-inflammatory and antiapoptotic effects of bone marrow and adipose-derived mesenchymal stem cells in acute alkaline corneal burnen_US
dc.typeArticleen_US

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