Expression of ESAT-6 antigen from mycobacterium tuberculosis in broccoli: An edible plant

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dc.authorid0000-0001-6767-2539en_US
dc.authorid0000-0002-9861-462Xen_US
dc.authorid0000-0001-6767-2539
dc.contributor.authorSaba, Kiran
dc.contributor.authorSameeullah, Muhammad
dc.contributor.authorAsghar, Asba
dc.contributor.authorGottschamel, Johanna
dc.contributor.authorLatif, Sara
dc.contributor.authorLossl, Andreas Gunter
dc.contributor.authorMirza, Bushra
dc.date.accessioned2021-06-23T19:54:40Z
dc.date.available2021-06-23T19:54:40Z
dc.date.issued2020
dc.departmentBAİBÜ, Ziraat Fakültesi, Bahçe Bitkileri Bölümüen_US
dc.description.abstractTuberculosis (TB) is one of the major infectious diseases caused by Mycobacterium tuberculosis. The development of an effective and economical vaccine for controlling TB is essential especially for developing countries. Edible plants can serve as biofactories to produce vaccine antigens. In this study, 6 kDa early secretory antigenic target (ESAT-6) of M. tuberculosis was expressed in Brassica oleracea var. italica via Agrobacterium-mediated transformation to facilitate oral delivery of antigen. ESAT-6 gene was cloned using Gateway (R) cloning strategy. Transformation and presence of transgene was confirmed through PCR. Expression level of transgene was calculated via quantitative real-time PCR (qRT-PCR) and the maximum integrated transgene number was two. Maximum amount of total soluble fraction of ESAT-6 was evaluated by immunoblotting, estimated to accumulate up to 0.5% of total soluble protein. The recombinant ESAT-6 protein was further purified and detected using silver staining and Western blotting. ESAT-6 protein induced humoral immune response in mice immunized orally and subcutaneously. The expression of M. tuberculosis antigen in edible plants could aid in the development of cost-effective and oral delivery of an antigen-based subunit vaccine against TB. To the best our knowledge, it is the first report of expression of a vaccine antigen in broccoli.en_US
dc.identifier.doi10.1002/bab.1867
dc.identifier.endpage157en_US
dc.identifier.issn0885-4513
dc.identifier.issn1470-8744
dc.identifier.issue1en_US
dc.identifier.pmid31898361en_US
dc.identifier.scopus2-s2.0-85077841844en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage148en_US
dc.identifier.urihttps://doi.org/10.1002/bab.1867
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10610
dc.identifier.volume67en_US
dc.identifier.wosWOS:000505486400001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSameeullah, Muhammad
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofBiotechnology And Applied Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAgrobacterium-mediated Transformationen_US
dc.subjectEdible Vaccinesen_US
dc.subjectESAT-6en_US
dc.subjectGateway (R) cloningen_US
dc.subjectHumoral Immune Responseen_US
dc.subjectMycobacterium Tuberculosisen_US
dc.titleExpression of ESAT-6 antigen from mycobacterium tuberculosis in broccoli: An edible planten_US
dc.typeArticleen_US

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