Evaluation of 4 methods for the serological diagnosis of Epstein-Barr virus infection using an immunofluorescence assay as the reference method

Basit Öğe Kaydı
dc.authorid0000-0002-9026-4485en_US
dc.contributor.authorKoçoğlu, Mücahide Esra
dc.contributor.authorTaş, Tekin
dc.contributor.authorMengeloğlu, Fırat Zafer
dc.contributor.authorÖzsoy, Şeyda
dc.contributor.authorBucak, Özlem
dc.date.accessioned2021-06-23T19:35:17Z
dc.date.available2021-06-23T19:35:17Z
dc.date.issued2014
dc.departmentBAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground/aim: Tests specific for VCA IgM, VCA IgG, and EBNA IgG are used to diagnose Epstein-Barr virus (EBV) infections and interpret disease status. The immunofluorescence assay (IFA) is accepted as the "gold standard" test. The purpose of this study was to evaluate the performance of 4 methods in comparison with IFA. Materials and methods: In total, 101 serum samples were obtained from clinically suspected cases of EBV infection between May 2010 and May 2012 and evaluated by IFA. All serum samples were analyzed by an immunoblot assay, enzyme-linked fluorescent assay (ELFA), enzyme immunoassay (EIA), and immunochromatographic assay (ICA). Results: ELFA and ICA results were in good agreement with IFA for the detection of VCA IgM, VCA IgG, and EBNA IgG. The results of the immunoblot assay agreed less well with IFA for EBNA IgG, while EIA results were not in agreement with IFA for EBNA IgG or VCA IgM. Conclusion: Among the tests studied, ELFA and ICA appear to be suitable methods for the diagnosis and staging of EBV when considering cost-effectiveness, turnaround times, need for a specialist, and IFA concordance.en_US
dc.identifier.doi10.3906/sag-1308-38
dc.identifier.endpage919en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue6en_US
dc.identifier.pmid25552141en_US
dc.identifier.scopus2-s2.0-84908067979en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage914en_US
dc.identifier.urihttps://doi.org/10.3906/sag-1308-38
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7757
dc.identifier.volume44en_US
dc.identifier.wosWOS:000344155900002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKoçoğlu, Mücahide Esra
dc.institutionauthorTaş, Tekin
dc.institutionauthorMengeloğlu, Fırat Zafer
dc.institutionauthorÖzsoy, Şeyda
dc.institutionauthorBucak, Özlem
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEpstein-Barr Virusen_US
dc.subjectELISAen_US
dc.subjectImmunoblot Assayen_US
dc.subjectImmunochromatographic Assayen_US
dc.titleEvaluation of 4 methods for the serological diagnosis of Epstein-Barr virus infection using an immunofluorescence assay as the reference methoden_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
mucahide-esra-kocoglu.pdf
Boyut:
84.31 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam metin/Full text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Temel Tıp Bilimleri Bölümü Koleksiyonu