Somatic embryogenesis, pigment accumulation, and synthetic seed production in Digitalis davisiana Heywood

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dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0003-0060-259X
dc.authorid0000-0003-2424-4803
dc.contributor.authorVerma, Sandeep Kumar
dc.contributor.authorŞahin, Günce
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:43:41Z
dc.date.available2021-06-23T19:43:41Z
dc.date.issued2016
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractDigitalis davisiana, commonly called Alanya foxglove, from Turkey, is an important medicinal herb as the main source of cardiac glycosides, cardenolides, anthraquinones, etc. It is also known in the Indian Medicine for treatment of wounds and burns. It has ornamental value as well. Overexploitation of D. davisiana has led this species to be declared protected, and thereby encouraged various methods for its propagation. In this study, an optimized and efficient plant tissue culture protocol was established using cotyledonary leaf, hypocotyl and root explants of D. davisiana. Callus tissues were obtained from the cotyledonary leaf, hypocotyl and root segments cultured on Murashige and Skoog's (MS) medium containing different plant growth regulators. The maximum number of somatic embryos were achieved by the MS medium containing 6-benzyladenine (1.0 mg/L BAP) or 2,4-dichlorophenoxy acetic acids (0.1 mg/L 2,4-D), which produced an average of 8.3 +/- 1.5 or 5.3 +/- 1.5 embryos per cotyledonary leaf, respectively. After 3 wk of culture in MS medium supplemented with 1.0 mg/L 2,4-D, callus showed a clear accumulation of orange pigmentation. Shoot regeneration was remarkably higher (14.3 indirect shoots) in a combination of alpha-naphthalene acetic acid (0.25 mg/L NAA) plus 3.0 mg/L BAP than 2.0 mg/L zeatin (10.3 +/- 0.5 direct shoots) alone. The shoots were successfully rooted on MS medium supplemented with NAA (0.1-1.0 mg/L). In addition, synthetic seeds were produced by encapsulating shoot tips in 4% sodium alginate solution. Maximum conversion frequency of 76.6% was noted from encapsulated shoot tips cultured on 0.25 mg/L NAA with 1.0 mg/L BAP. The encapsulated shoot tips could be stored up to 60 days at 4 degrees C. Regenerated plantlets of D. davisiana were successfully acclimatized and transferred to soil. This study has demonstrated successful preservation of elite genotypes of D. davisiana.en_US
dc.identifier.endpage253en_US
dc.identifier.issn0019-5189
dc.identifier.issn0975-1009
dc.identifier.issue4en_US
dc.identifier.pmid27295921en_US
dc.identifier.scopus2-s2.0-84964077788en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage245en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8832
dc.identifier.urihttps://www.webofscience.com/wos/woscc/full-record/WOS:000373869800003
dc.identifier.volume54en_US
dc.identifier.wosWOS:000373869800003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorGürel, Ekrem
dc.institutionauthorŞahin, Günce
dc.language.isoenen_US
dc.publisherNatl Inst Science Communication-Niscairen_US
dc.relation.ispartofIndian Journal Of Experimental Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlanya Foxgloveen_US
dc.subjectAnthraquinonesen_US
dc.subjectAuxinen_US
dc.subjectCallus Initiationen_US
dc.subjectCardenolidesen_US
dc.subjectHerbalen_US
dc.subjectMicropropagationen_US
dc.titleSomatic embryogenesis, pigment accumulation, and synthetic seed production in Digitalis davisiana Heywooden_US
dc.typeArticleen_US

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