Absence of the mecC gene in methicillin-resistant staphylococcus aureus isolated from various clinical samples: The first multi-centered study in Turkey

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dc.authorid0000-0002-2605-1282en_US
dc.authorid0000-0002-4144-2409en_US
dc.authorid0000-0003-3724-8491en_US
dc.authorid0000-0002-1522-6083en_US
dc.authorid0000-0002-7057-2501
dc.contributor.authorÇıkman, Aytekin
dc.contributor.authorAydın, Merve
dc.contributor.authorGülhan, Barış
dc.contributor.authorKarakeçili, Faruk
dc.contributor.authorKurtoğlu, Muhammet Güzel
dc.contributor.authorYüksekkaya, Şerife
dc.contributor.authorParlak, Mehmet
dc.date.accessioned2021-06-23T19:51:26Z
dc.date.available2021-06-23T19:51:26Z
dc.date.issued2019
dc.departmentBAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground: mecA is a predefined gene causing methicillin resistance in Staphylococcus aureus (S. aureus) isolates; however, it has been shown that some methicillin-resistant S. aureus (MRSA) strains do not carry this gene. Recently, in isolates found to be MRSA-positive but mecA-negative, a new resistance gene called mecC, which is a homolog of mecA, has been reported. This study aimed to investigate the mecC and mecA genes in MRSA strains isolated from different geographic regions in Turkey. Methods: The sample of the study consisted of 494 MRSA strains isolated from seven geographical regions in Turkey between 2013 and 2016. The strains were obtained from 17 centers, comprising 13 university hospitals, three education and research hospitals, and one state hospital. Methicillin resistance in S. aureus strains was determined using the agar disk diffusion method with a cefoxitin disk and the agar dilution method with oxacillin. The mecC and mecA genes in MRSA strains was investigated by Polymerase Chain Reaction (PCR). Results: Of the MRSA strains investigated, 47.9% were isolated from intensive care units. Concerning sample type, 36.7% were detected in the respiratory tract (tracheal aspirate, sputum, etc.), 24.8% in blood, 18.7% in skin and soft tissues, 9.3% in nasal swabs, 5.4% in urine, 4.1% in ears, and 1% in sterile body fluid. Using PCR, mecC was not identified in any of the S. aureus strains isolated from different clinical microbiology laboratories. mecA gene positivity was found in 315 of the MRSA strains (63.8%). Staphylococcal Cassette Chromosome mec ( SCCmec) type was identified in 232 strains (46.9%), of which 136 (58.7%) were type II, 75 (32.4%) were type IV, 12 (5.1%) were type Mb, six (2.5%) were type I, and three (1.3%) were type III. Conclusion: This is the first multi-centered study to investigate MRSA strains isolated from different regions in Turkey. The mecC gene was not detected in any of the MRSA strains. We believe that this study will constitute an important basis for monitoring possible future changes. (C) 2019 The Authors. Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences.en_US
dc.identifier.doi10.1016/j.jiph.2019.01.063
dc.identifier.endpage533en_US
dc.identifier.issn1876-0341
dc.identifier.issn1876-035X
dc.identifier.issue4en_US
dc.identifier.pmid30745200en_US
dc.identifier.scopus2-s2.0-85061095396en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage528en_US
dc.identifier.urihttps://doi.org/10.1016/j.jiph.2019.01.063
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9985
dc.identifier.volume12en_US
dc.identifier.wosWOS:000473414800014en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKurtoğlu, Muhammet Güzel
dc.language.isoenen_US
dc.publisherElsevier Science Londonen_US
dc.relation.ispartofJournal Of Infection And Public Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMethicillin Resistanceen_US
dc.subjectStaphylococcus Aureusen_US
dc.subjectMecCen_US
dc.subjectMecAen_US
dc.subjectTurkeyen_US
dc.titleAbsence of the mecC gene in methicillin-resistant staphylococcus aureus isolated from various clinical samples: The first multi-centered study in Turkeyen_US
dc.typeArticleen_US

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