The effects of oxidative stress on phenolic composition and antioxidant metabolism in callus culture of common daisy

Basit Öğe Kaydı
dc.authorid0000-0001-5245-6294
dc.authorid0000-0003-0060-259X
dc.authorid0000-0001-9617-6673
dc.contributor.authorKarakaş, Fatma Pehlivan
dc.contributor.authorCingöz, Günce Şahin
dc.contributor.authorTürker, Arzu Uçar
dc.date.accessioned2021-06-23T19:44:14Z
dc.date.available2021-06-23T19:44:14Z
dc.date.issued2016
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.description.abstractBackground: Exogenous pretreatment of oxidative stress on callus cultures of Bellis perennis L. (common daisy) induced catalase (CAT), superoxide dismutase (SOD), total phenolic, total flavonoid, total protein and selected commercial phenolic compounds production and accumulation. Materials and Methods: The callus culture obtained from B. perennis pedicel explants was incubated on Murashige and Skoog medium (MS) containing 10 mM H2O2 or 0 mM H2O2 (for control group) for 10 hours. Twenty phenolic compounds (apigenin, caffeic acid, p-coumaric acid, gallic acid, genistein, kaempferol, luteolin, myricetin, procyanidin-C1, quercetin, rutin hydrate, vanillic acid, ferulic acid, salicylic acid, sinapic acid, chlorogenic acid, hesperedin, naringenin, rosmarinic acid and isorhamnetin) were detected by LC-ESI-MS/MS analysis in methanolic extracts of 10 mM H2O2 and control treatments. Results: A predominant phenolic compound was chlorogenic acid followed by rutin hydrate, caffeic acid, luteoline, isorhamnetin, quercetin, myricetin, apigenin, p-coumaric acid and kaempferol. No gallic acid, genistein, procyanidin-C1, vanillic acid, sinapic acid, hesperidin and naringenin were detected in H2O2 treatment and control groups of B. perennis. The total phenolic contents estimated were in the order of H2O2 treatment (285.36 mu g/g dw) and control (220.79 mu g/g dw) groups. The biosynthesis and accumulation of kaempferol, myricetin, quercetin and isorhamnetin were only determined in H2O2 treatment callus materials. The H2O2 pretreatment clearly showed in a raise in enzymatic and non-enzymatic antioxidant activities. Finally, a significant positive correlation between phenolic accumulation and comprehensive activities of CAT, SOD, total phenolic, total flavonoid and proline was accessible. Conclusion: The present results suggest that using H2O2 as an elicitor or a stimulant plays a significant enhancement role in special phenolic molecules biosynthesis and activation of antioxidant metabolism on callus cultures of B. perennis.en_US
dc.identifier.doi10.21010/ajtcam.v13i4.6
dc.identifier.endpage41en_US
dc.identifier.issn0189-6016
dc.identifier.issue4en_US
dc.identifier.pmid28852718en_US
dc.identifier.scopus2-s2.0-85006109796en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage34en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8946
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566151/
dc.identifier.volume13en_US
dc.identifier.wosWOS:000387255300005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKarakaş, Fatma Pehlivan
dc.institutionauthorCingöz, Günce Şahin
dc.institutionauthorTürker, Arzu Uçar
dc.language.isoenen_US
dc.publisherAfrican Networks Ethnomedicinesen_US
dc.relation.ispartofAfrican Journal Of Traditional Complementary And Alternative Medicinesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBellis Perennisen_US
dc.subjectPedicel Explanten_US
dc.subjectCallus Cultureen_US
dc.subjectSODen_US
dc.subjectCATen_US
dc.subjectAntioxidant Activityen_US
dc.subjectHydrogen Peroxideen_US
dc.titleThe effects of oxidative stress on phenolic composition and antioxidant metabolism in callus culture of common daisyen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
fatma-pehlivan-karakas.pdf
Boyut:
669.33 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Biyoloji Bölümü Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Tarla Bitkileri Bölümü Koleksiyonu